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J Bacteriol, February 1998, p. 556-562, Vol. 180, No. 3
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Intracellular Glucose Concentration in Derepressed Yeast Cells Consuming Glucose Is High Enough To Reduce the Glucose Transport Rate by 50%

Bas Teusink,1,2 Jasper A. Diderich,1 Hans V. Westerhoff,1,3 Karel van Dam,1 and Michael C. Walsh1,*

E. C. Slater Institute, BioCentrum, University of Amsterdam, NL-1018 TV Amsterdam,1 and Department of Microbial Physiology, Faculty of Biology, Vrije Universiteit, NL-1081 HV Amsterdam,3 The Netherlands, and Department of Biomolecular Sciences, UMIST, Manchester M60 1QD, United Kingdom2

Received 30 October 1997/Accepted 3 December 1997

In Saccharomyces cerevisiae cells exhibiting high-affinity glucose transport, the glucose consumption rate at extracellular concentrations above 10 mM was only half of the zero trans-influx rate. To determine if this regulation of glucose transport might be a consequence of intracellular free glucose we developed a new method to measure intracellular glucose concentrations in cells metabolizing glucose, which compares glucose stereoisomers to correct for adhering glucose. The intracellular glucose concentration was 1.5 mM, much higher than in most earlier reports. We show that for the simplest model of a glucose carrier, this concentration is sufficient to reduce the glucose influx by 50%. We conclude that intracellular glucose is the most likely candidate for the observed regulation of glucose import and hence glycolysis. We discuss the possibility that intracellular glucose functions as a primary signal molecule in these cells.

* Corresponding author. Mailing address: E. C. Slater Institute, BioCentrum, University of Amsterdam, Plantage Muidergracht 12, NL-1018 TV Amsterdam, The Netherlands. Phone: 31 20 5255069. Fax: 31 20 5255124. E-mail: Mike.Walsh{at}chem.uva.nl.




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