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J Bacteriol, February 1998, p. 642-646, Vol. 180, No. 3
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Demonstration of the Key Role Played by the PufX Protein in the Functional and Structural Organization of Native and Hybrid Bacterial Photosynthetic Core Complexes

Timothy K. Fulcher,1 J. Thomas Beatty,2 and Michael R. Jones1,*

Robert Hill Institute for Photosynthesis and Krebs Institute for Biomolecular Research, University of Sheffield, Sheffield, United Kingdom,1 and Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada2

Received 18 July 1997/Accepted 21 November 1997

The role of a component of the bacterial photosystem, the PufX protein, was examined by heterologous expression of the pufX gene from Rhodobacter capsulatus in a strain of R. sphaeroides that lacks the native pufX gene. The strain of R. sphaeroides containing the R. capsulatus PufX protein was capable of efficient transduction of light energy despite a low degree of sequence conservation between the PufX proteins from the two species. The organization of the hybrid reaction center/LH1 photosystem in strains of R. sphaeroides containing the R. capsulatus LH1 antenna complex was affected differently by the R. sphaeroides and R. capsulatus PufX proteins. We discuss the implications of our findings for the role of the PufX protein in organizing the bacterial photosystem for efficient transduction of light energy.

* Corresponding author. Mailing address: Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2UH, United Kingdom. Phone: 114-2224234. Fax: 114-2728697. E-mail: m.jones{at}sheffield.ac.uk.




This article has been cited by other articles:

  • Cogdell, R. J., Isaacs, N. W., Howard, T. D., McLuskey, K., Fraser, N. J., Prince, S. M. (1999). How Photosynthetic Bacteria Harvest Solar Energy. J. Bacteriol. 181: 3869-3879 [Full Text]  
  • Hebermehl, M., Klug, G. (1998). Effect of Oxygen on Translation and Posttranslational Steps in Expression of Photosynthesis Genes in Rhodobacter capsulatus. J. Bacteriol. 180: 3983-3987 [Abstract] [Full Text]  


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