This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Margot, P. Articles by Karamata, D. Search for Related Content PubMed PubMed Citation Articles by Margot, P. Articles by Karamata, D.

 Previous Article  |  Next Article 

J Bacteriol, February 1998, p. 749-752, Vol. 180, No. 3
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The lytE Gene of Bacillus subtilis 168 Encodes a Cell Wall Hydrolase

Philippe Margot,¹ Michael Wahlen,² Ahmad Gholamhuseinian,^2, Patrick Piggot,² and Dimitri Karamata^1,*

Institut de Génétique et de Biologie Microbiennes, CH-1005 Lausanne, Switzerland,¹ and Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140²

Received 2 September 1997/Accepted 1 December 1997

Bacillus subtilis cell wall-bound protein CWBP33 is encoded by lytE, a gene expressed during the exponential growth phase. Sequence analysis of LytE, a 33-kDa protein, reveals two domains. The N-terminal domain contains a threefold-repeated motif common to several peptidoglycan binding proteins, while the C-terminal domain, probably carrying the catalytic activity, has homology with certain exoproteins. Zymographs unambiguously reveal that the absence of CWBP33, due to inactivation of lytE, is accompanied by the loss of a lytic activity. In lytE mutants, the cell autolysis rate is significantly decreased, although autolysis of corresponding, purified cell walls does not seem to be affected.

---

^* Corresponding author. Mailing address: Institut de Génétique et de Biologie Microbiennes, Rue César-Roux 19, CH-1005 Lausanne, Switzerland. Phone: 41 21 3206075. Fax: 41 21 3206078. E-mail: dimitri.karamata{at}igbm.unil.ch

Present address: Kerman University of Medical Sciences, Kerman, Iran.

This article has been cited by other articles:

• Haiser, H. J., Yousef, M. R., Elliot, M. A. (2009). Cell Wall Hydrolases Affect Germination, Vegetative Growth, and Sporulation in Streptomyces coelicolor. J. Bacteriol. 191: 6501-6512 [Abstract] [Full Text]  
• Frias, M. J., Melo-Cristino, J., Ramirez, M. (2009). The Autolysin LytA Contributes to Efficient Bacteriophage Progeny Release in Streptococcus pneumoniae. J. Bacteriol. 191: 5428-5440 [Abstract] [Full Text]  
• Andre, G., Leenhouts, K., Hols, P., Dufrene, Y. F. (2008). Detection and Localization of Single LysM-Peptidoglycan Interactions. J. Bacteriol. 190: 7079-7086 [Abstract] [Full Text]  
• Redko, Y., Courtin, P., Mezange, C., Huard, C., Chapot-Chartier, M.-P. (2007). Lactococcus lactis Gene yjgB Encodes a {gamma}-D-Glutaminyl-L-Lysyl- Endopeptidase Which Hydrolyzes Peptidoglycan. Appl. Environ. Microbiol. 73: 5825-5831 [Abstract] [Full Text]  
• Machata, S., Hain, T., Rohde, M., Chakraborty, T. (2005). Simultaneous Deficiency of both MurA and p60 Proteins Generates a Rough Phenotype in Listeria monocytogenes. J. Bacteriol. 187: 8385-8394 [Abstract] [Full Text]  
• Steen, A., Palumbo, E., Deghorain, M., Cocconcelli, P. S., Delcour, J., Kuipers, O. P., Kok, J., Buist, G., Hols, P. (2005). Autolysis of Lactococcus lactis Is Increased upon D-Alanine Depletion of Peptidoglycan and Lipoteichoic Acids. J. Bacteriol. 187: 114-124 [Abstract] [Full Text]  
• Tjalsma, H., Antelmann, H., Jongbloed, J. D.H., Braun, P. G., Darmon, E., Dorenbos, R., Dubois, J.-Y. F., Westers, H., Zanen, G., Quax, W. J., Kuipers, O. P., Bron, S., Hecker, M., van Dijl, J. M. (2004). Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome. Microbiol. Mol. Biol. Rev. 68: 207-233 [Abstract] [Full Text]  
• Yamamoto, H., Kurosawa, S.-i., Sekiguchi, J. (2003). Localization of the Vegetative Cell Wall Hydrolases LytC, LytE, and LytF on the Bacillus subtilis Cell Surface and Stability of These Enzymes to Cell Wall-Bound or Extracellular Proteases. J. Bacteriol. 185: 6666-6677 [Abstract] [Full Text]  
• Steen, A., Buist, G., Leenhouts, K. J., Khattabi, M. E., Grijpstra, F., Zomer, A. L., Venema, G., Kuipers, O. P., Kok, J. (2003). Cell Wall Attachment of a Widely Distributed Peptidoglycan Binding Domain Is Hindered by Cell Wall Constituents. J. Biol. Chem. 278: 23874-23881 [Abstract] [Full Text]  
• Tjalsma, H., Bolhuis, A., Jongbloed, J. D. H., Bron, S., van Dijl, J. M. (2000). Signal Peptide-Dependent Protein Transport in Bacillus subtilis: a Genome-Based Survey of the Secretome. Microbiol. Mol. Biol. Rev. 64: 515-547 [Abstract] [Full Text]  
• Chen, Y., Fukuoka, S., Makino, S. (2000). A Novel Spore Peptidoglycan Hydrolase of Bacillus cereus: Biochemical Characterization and Nucleotide Sequence of the Corresponding Gene, sleL. J. Bacteriol. 182: 1499-1506 [Abstract] [Full Text]  
• Smith, T. J., Blackman, S. A., Foster, S. J. (2000). Autolysins of Bacillus subtilis: multiple enzymes with multiple functions. Microbiology 146: 249-262 [Full Text]  
• Nugroho, F. A., Yamamoto, H., Kobayashi, Y., Sekiguchi, J. (1999). Characterization of a New Sigma-K-Dependent Peptidoglycan Hydrolase Gene That Plays a Role in Bacillus subtilis Mother Cell Lysis. J. Bacteriol. 181: 6230-6237 [Abstract] [Full Text]  
• Kodama, T., Takamatsu, H., Asai, K., Kobayashi, K., Ogasawara, N., Watabe, K. (1999). The Bacillus subtilis yaaH Gene Is Transcribed by SigE RNA Polymerase during Sporulation, and Its Product Is Involved in Germination of Spores. J. Bacteriol. 181: 4584-4591 [Abstract] [Full Text]  
• Ohnishi, R., Ishikawa, S., Sekiguchi, J. (1999). Peptidoglycan Hydrolase LytF Plays a Role in Cell Separation with CwlF during Vegetative Growth of Bacillus subtilis. J. Bacteriol. 181: 3178-3184 [Abstract] [Full Text]