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J Bacteriol, February 1998, p. 1002-1007, Vol. 180, No. 4
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Relationships among the O-Antigen Gene Clusters of Salmonella enterica Groups B, D1, D2, and D3

Heather Curd, Dan Liu, and Peter R. Reeves*

Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia

Received 21 July 1997/Accepted 6 December 1997

The O antigen is an important cell wall antigen of gram-negative bacteria, and the genes responsible for its biosynthesis are located in a gene cluster. We have cloned and sequenced the DNA segment unique to the O-antigen gene cluster of Salmonella enterica group D3. This segment includes a novel O-antigen polymerase gene (wzyD3). The polymerase gives alpha (1right-arrow6) linkages but has no detectable sequence similarity to that of group D2, which confers the same linkage. We find the remnant of a D3-like wzy gene in the O-antigen gene clusters of groups D1 and B and suggest that this is the original wzy gene of these O-antigen gene clusters.

* Corresponding author. Mailing address: Department of Microbiology (G08), The University of Sydney, NSW 2006, Australia. Phone: 61-2-9351 2536. Fax: 61-2-9351 4571. E-mail: reeves{at}angis.usyd.edu.au.




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