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J Bacteriol, February 1998, p. 767-772, Vol. 180, No. 4
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Perturbation of Anion Balance during Inhibition of Growth of Escherichia coli by Weak Acids

Andrew J. Roe,1 Debra McLaggan,1 Ian Davidson,1 Conor O'Byrne,2,dagger and Ian R. Booth1,*

Department of Molecular and Cell Biology, Institute of Medical Science, Foresterhill, Aberdeen, AB25 2ZD,1 and Microbiology Department, Unilever Research, Colworth House, Sharnbrook, Bedford, MK44 1LQ,2 United Kingdom

Received 15 September 1997/Accepted 16 December 1997

During inhibition of cell growth by weak acids, there is substantial accumulation of the weak acid anions in the cytoplasm. This study was undertaken to determine the impact of anion accumulation on cellular pools. At pH 6, growth in the presence of 8 mM acetate led to an internal pool of greater than 240 mM acetate anion and resulted in reduced levels of glutamate in the cell, but there were no significant changes in K+ and Na+ levels. At low osmolarity, the change in the glutamate pool compensated for only a small fraction of the accumulated acetate anion. However, at high osmolarity, glutamate compensated for over half of the accumulated acetate. Recovery of the normal cytoplasmic pH after the removal of acetate was dependent on the synthesis of glutamate.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Institute of Medical Science, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom. Phone: (44-1224) 273152. Fax: (44-1224) 273144. E-mail: gen118{at}abdn.ac.uk.

dagger Present address: Department of Molecular and Cell Biology, Institute of Medical Science, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom.




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