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J Bacteriol, February 1998, p. 909-913, Vol. 180, No. 4
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Major Outer Membrane Protein of Rahnella aquatilis Functions as a Porin and Root Adhesin

Wafa Achouak,1,* Jean-Marie Pages,2 Rene De Mot,3 Gerard Molle,4 and Thierry Heulin1

Laboratoire d'Ecologie Microbienne de la Rhizosphère, DSV-DEVM, UMR 163 CNRS-CEA, CEA Cadarache, F-13108 Saint Paul lez Durance,1 INSERM CJF 9606, Enveloppe Bactérienne, Antibiotiques et Colonisation, Faculté de Médecine, F-13385 Marseille Cedex 5,2 and UMR 6522 CNRS IFRMP 23, Faculté des Sciences de Rouen, F-76821 Mont-Saint Aignan Cedex,4 France, and F. A. Janssens Laboratory of Genetics, Katolieke Universiteit Leuven, B-3001 Heverlee, Belgium3

Received 28 July 1997/Accepted 16 December 1997

A 38-kDa major outer membrane protein (OMP) was isolated from the nitrogen-fixing enterobacterium Rahnella aquatilis CF3. This protein exists as a stable trimer in the presence of 2% sodium dodecyl sulfate at temperatures below 60°C. Single channel experiments showed that this major OMP of R. aquatilis CF3 is able to form pores in the planar lipid membrane. Two oligonucleotides encoding the N-terminal portion of the 38-kDa OMP and C-terminal portion of OmpC were used to amplify the 38-kDa gene by PCR. The deduced amino acid sequence showed a strong homology with Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, and Serratia marcescens OmpC sequences, except loops L6 and L7, which are postulated to be cell surface exposed. On the basis of the OmpF-PhoE three-dimensional structure, it seems likely that this 38-kDa organizes three 16-strand beta -barrel subunits. The relationship between the structure and the double functionality of this protein as porin and as a root adhesin is discussed.

* Corresponding author. Mailing address: Laboratoire d'Ecologie Microbienne de la Rhizosphère, DSV-DEVM, UMR 163 CNRS-CEA, CEA Cadarache, F-13108 Saint Paul lez Durance, France. Phone: (33) 44225 4961. Fax: (33) 44225 6648. E-mail: achouak{at}soumman.cad.cea.fr.




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