This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aono, R.
Right arrow Articles by Yamamoto, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aono, R.
Right arrow Articles by Yamamoto, M.

 Previous Article  |  Next Article 

J Bacteriol, February 1998, p. 938-944, Vol. 180, No. 4
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Involvement of Outer Membrane Protein TolC, a Possible Member of the mar-sox Regulon, in Maintenance and Improvement of Organic Solvent Tolerance of Escherichia coli K-12

Rikizo Aono,* Norihiko Tsukagoshi, and Mami Yamamoto

Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226, Japan

Received 2 September 1997/Accepted 6 December 1997

Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS. A possible mar-rob-sox box sequence was found upstream of the tolC gene. These findings suggest that tolC is a member of the mar-sox regulon responsive to stress conditions. When a defective tolC gene was transferred to n-hexane- or cyclohexane-tolerant strains by P1 transduction, the organic solvent tolerance level was lowered dramatically to the decane-tolerant and nonane-sensitive level. The tolerance level was restored by transformation of the transductants with a wild-type tolC gene. Therefore, it is evident that TolC is essential for E. coli to maintain organic solvent tolerance.

* Corresponding author. Mailing address: Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226, Japan. Phone: 81-45-924-5776. Fax: (81) 45-924-5819. E-mail: raono{at}bio.titech.ac.jp.




This article has been cited by other articles:

  • Sun, X., Dennis, J. J. (2009). A Novel Insertion Sequence Derepresses Efflux Pump Expression and Preadapts Pseudomonas putida S12 for Extreme Solvent Stress. J. Bacteriol. 191: 6773-6777 [Abstract] [Full Text]  
  • Duval, V., Nicoloff, H., Levy, S. B. (2009). Combined Inactivation of lon and ycgE Decreases Multidrug Susceptibility by Reducing the Amount of OmpF Porin in Escherichia coli. Antimicrob. Agents Chemother. 53: 4944-4948 [Abstract] [Full Text]  
  • Rosner, J. L., Martin, R. G. (2009). An Excretory Function for the Escherichia coli Outer Membrane Pore TolC: Upregulation of marA and soxS Transcription and Rob Activity Due to Metabolites Accumulated in tolC Mutants. J. Bacteriol. 191: 5283-5292 [Abstract] [Full Text]  
  • Gil, F., Hernandez-Lucas, I., Polanco, R., Pacheco, N., Collao, B., Villarreal, J. M., Nardocci, G., Calva, E., Saavedra, C. P. (2009). SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene. Microbiology 155: 2490-2497 [Abstract] [Full Text]  
  • Yamanaka, H., Kobayashi, H., Takahashi, E., Okamoto, K. (2008). MacAB Is Involved in the Secretion of Escherichia coli Heat-Stable Enterotoxin II. J. Bacteriol. 190: 7693-7698 [Abstract] [Full Text]  
  • Tatsumi, R., Wachi, M. (2008). TolC-Dependent Exclusion of Porphyrins in Escherichia coli. J. Bacteriol. 190: 6228-6233 [Abstract] [Full Text]  
  • de Cristobal, R. E., Vincent, P. A., Salomon, R. A. (2008). A Combination of sbmA and tolC Mutations in Escherichia coli K-12 Tn10-Carrying Strains Results in Hypersusceptibility to Tetracycline. J. Bacteriol. 190: 1491-1494 [Abstract] [Full Text]  
  • DeVito, J. A. (2008). Recombineering with tolC as a Selectable/Counter-selectable Marker: remodeling the rRNA Operons of Escherichia coli. Nucleic Acids Res 36: e4-e4 [Abstract] [Full Text]  
  • Bleuel, C., Grosse, C., Taudte, N., Scherer, J., Wesenberg, D., Krauss, G. J., Nies, D. H., Grass, G. (2005). TolC Is Involved in Enterobactin Efflux across the Outer Membrane of Escherichia coli. J. Bacteriol. 187: 6701-6707 [Abstract] [Full Text]  
  • Teran, W., Felipe, A., Segura, A., Rojas, A., Ramos, J.-L., Gallegos, M.-T. (2003). Antibiotic-Dependent Induction of Pseudomonas putida DOT-T1E TtgABC Efflux Pump Is Mediated by the Drug Binding Repressor TtgR. Antimicrob. Agents Chemother. 47: 3067-3072 [Abstract] [Full Text]  
  • Eguchi, Y., Oshima, T., Mori, H., Aono, R., Yamamoto, K., Ishihama, A., Utsumi, R. (2003). Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli. Microbiology 149: 2819-2828 [Abstract] [Full Text]  
  • Franke, S., Grass, G., Rensing, C., Nies, D. H. (2003). Molecular Analysis of the Copper-Transporting Efflux System CusCFBA of Escherichia coli. J. Bacteriol. 185: 3804-3812 [Abstract] [Full Text]  
  • Gayet, S., Chollet, R., Molle, G., Pages, J.-M., Chevalier, J. (2003). Modification of Outer Membrane Protein Profile and Evidence Suggesting an Active Drug Pump in Enterobacter aerogenes Clinical Strains. Antimicrob. Agents Chemother. 47: 1555-1559 [Abstract] [Full Text]  
  • Abe, S., Okutsu, T., Nakajima, H., Kakuda, N., Ohtsu, I., Aono, R. (2003). n-Hexane sensitivity of Escherichia coli due to low expression of imp/ostA encoding an 87 kDa minor protein associated with the outer membrane. Microbiology 149: 1265-1273 [Abstract] [Full Text]  
  • Grkovic, S., Brown, M. H., Skurray, R. A. (2002). Regulation of Bacterial Drug Export Systems. Microbiol. Mol. Biol. Rev. 66: 671-701 [Abstract] [Full Text]  
  • Michan, C., Manchado, M., Pueyo, C. (2002). SoxRS Down-Regulation of rob Transcription. J. Bacteriol. 184: 4733-4738 [Abstract] [Full Text]  
  • Helling, R. B., Janes, B. K., Kimball, H., Tran, T., Bundesmann, M., Check, P., Phelan, D., Miller, C. (2002). Toxic Waste Disposal in Escherichia coli. J. Bacteriol. 184: 3699-3703 [Abstract] [Full Text]  
  • Shyu, J. B. H., Lies, D. P., Newman, D. K. (2002). Protective Role of tolC in Efflux of the Electron Shuttle Anthraquinone-2,6-Disulfonate. J. Bacteriol. 184: 1806-1810 [Abstract] [Full Text]  
  • Rosner, J. L., Dangi, B., Gronenborn, A. M., Martin, R. G. (2002). Posttranscriptional Activation of the Transcriptional Activator Rob by Dipyridyl in Escherichia coli. J. Bacteriol. 184: 1407-1416 [Abstract] [Full Text]  
  • Yassien, Mahmoud. A., Ewis, H. E., Lu, C.-D., Abdelal, A. T. (2002). Molecular Cloning and Characterization of the Salmonella enterica Serovar Paratyphi B rma Gene, Which Confers Multiple Drug Resistance in Escherichia coli. Antimicrob. Agents Chemother. 46: 360-366 [Abstract] [Full Text]  
  • Diaz, E., Ferrandez, A., Prieto, M. A., Garcia, J. L. (2001). Biodegradation of Aromatic Compounds by Escherichia coli. Microbiol. Mol. Biol. Rev. 65: 523-569 [Abstract] [Full Text]  
  • Yamanaka, H., Izawa, H., Okamoto, K. (2001). Carboxy-Terminal Region Involved in Activity of Escherichia coli TolC. J. Bacteriol. 183: 6961-6964 [Abstract] [Full Text]  
  • Ramos-Gonzalez, M.-I., Godoy, P., Alaminos, M., Ben-Bassat, A., Ramos, J.-L. (2001). Physiological Characterization of Pseudomonas putida DOT-T1E Tolerance to p-Hydroxybenzoate. Appl. Environ. Microbiol. 67: 4338-4341 [Abstract] [Full Text]  
  • Segura, A., Duque, E., Hurtado, A., Ramos, J. L. (2001). Mutations in Genes Involved in the Flagellar Export Apparatus of the Solvent-Tolerant Pseudomonas putida DOT-T1E Strain Impair Motility and Lead to Hypersensitivity to Toluene Shocks. J. Bacteriol. 183: 4127-4133 [Abstract] [Full Text]  
  • Otto, K., Norbeck, J., Larsson, T., Karlsson, K.-A., Hermansson, M. (2001). Adhesion of Type 1-Fimbriated Escherichia coli to Abiotic Surfaces Leads to Altered Composition of Outer Membrane Proteins. J. Bacteriol. 183: 2445-2453 [Abstract] [Full Text]  
  • Kobayashi, K., Tsukagoshi, N., Aono, R. (2001). Suppression of Hypersensitivity of Escherichia coli acrB Mutant to Organic Solvents by Integrational Activation of the acrEF Operon with the IS1 or IS2 Element. J. Bacteriol. 183: 2646-2653 [Abstract] [Full Text]  
  • Koutsolioutsou, A., Martins, E. A., White, D. G., Levy, S. B., Demple, B. (2001). A soxRS-Constitutive Mutation Contributing to Antibiotic Resistance in a Clinical Isolate of Salmonella enterica (Serovar Typhimurium). Antimicrob. Agents Chemother. 45: 38-43 [Abstract] [Full Text]  
  • Piddock, L. J. V., White, D. G., Gensberg, K., Pumbwe, L., Griggs, D. J. (2000). Evidence for an Efflux Pump Mediating Multiple Antibiotic Resistance in Salmonella enterica Serovar Typhimurium. Antimicrob. Agents Chemother. 44: 3118-3121 [Abstract] [Full Text]  
  • Kopytek, S. J., Dyer, J. C. D., Knapp, G. S., Hu, J. C. (2000). Resistance to Methotrexate Due to AcrAB-Dependent Export from Escherichia coli. Antimicrob. Agents Chemother. 44: 3210-3212 [Abstract] [Full Text]  
  • Poole, K. (2000). Efflux-Mediated Resistance to Fluoroquinolones in Gram-Negative Bacteria. Antimicrob. Agents Chemother. 44: 2233-2241 [Full Text]  
  • Tsukagoshi, N., Aono, R. (2000). Entry into and Release of Solvents by Escherichia coli in an Organic-Aqueous Two-Liquid-Phase System and Substrate Specificity of the AcrAB-TolC Solvent-Extruding Pump. J. Bacteriol. 182: 4803-4810 [Abstract] [Full Text]  
  • Barbosa, T. M., Levy, S. B. (2000). Differential Expression of over 60 Chromosomal Genes in Escherichia coli by Constitutive Expression of MarA. J. Bacteriol. 182: 3467-3474 [Abstract] [Full Text]  
  • Kern, W. V., Oethinger, M., Jellen-Ritter, A. S., Levy, S. B. (2000). Non-Target Gene Mutations in the Development of Fluoroquinolone Resistance in Escherichia coli. Antimicrob. Agents Chemother. 44: 814-820 [Abstract] [Full Text]  
  • Mosqueda, G., Ramos, J.-L. (2000). A Set of Genes Encoding a Second Toluene Efflux System in Pseudomonas putida DOT-T1E Is Linked to the tod Genes for Toluene Metabolism. J. Bacteriol. 182: 937-943 [Abstract] [Full Text]  
  • Nesper, J., Blaß, J., Fountoulakis, M., Reidl, J. (1999). Characterization of the Major Control Region of Vibrio cholerae Bacteriophage K139: Immunity, Exclusion, and Integration. J. Bacteriol. 181: 2902-2913 [Abstract] [Full Text]  
  • Asako, H., Kobayashi, K., Aono, R. (1999). Organic Solvent Tolerance of Escherichia coli Is Independent of OmpF Levels in the Membrane. Appl. Environ. Microbiol. 65: 294-296 [Abstract] [Full Text]  
  • Li, X.-Z., Zhang, L., Poole, K. (1998). Role of the Multidrug Efflux Systems of Pseudomonas aeruginosa in Organic Solvent Tolerance. J. Bacteriol. 180: 2987-2991 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»