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J Bacteriol, February 1998, p. 998-1001, Vol. 180, No. 4
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of the Fucose Synthetase Gene in the Colanic Acid Gene Cluster of Escherichia coli K-12

Kanella Andrianopoulos, Lei Wang, and Peter R. Reeves*

Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia

Received 23 October 1997/Accepted 10 December 1997

GDP-L-fucose, the substrate for fucosyltransferases for addition of fucose to polysaccharides or glycoproteins in both procaryotes and eucaryotes, is made from GDP-D-mannose. L-Fucose is a component of bacterial surface antigens, including the extracellular polysaccharide colanic acid produced by most Escherichia coli strains. We previously sequenced the E. coli colanic acid gene cluster and identified one of the GDP-L-fucose biosynthetic pathway genes, gmd. We report here the identification of the gene (fcl), located downstream of gmd, encoding the fucose synthetase.

* Corresponding author. Mailing address: Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia. Phone: (612) 9351 2536. Fax: (612) 9351 4571. E-mail: reeves{at}angis.su.oz.au.




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