This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Velasco, A.
Right arrow Articles by Díaz, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Velasco, A.
Right arrow Articles by Díaz, E.

 Previous Article  |  Next Article 

J Bacteriol, March 1998, p. 1063-1071, Vol. 180, No. 5
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Genetic and Functional Analysis of the Styrene Catabolic Cluster of Pseudomonas sp. Strain Y2

Ana Velasco,1,2 Sergio Alonso,2 José L. García,1,* J. Perera,2 and Eduardo Díaz1

Department of Molecular Microbiology, Centro de Investigaciones Biológicas, CSIC, 28006 Madrid,1 and Department of Biochemistry and Molecular Biology, Facultad de Biología, Universidad Complutense, 28040 Madrid,2 Spain

Received 6 October 1997/Accepted 6 December 1997

The chromosomal region of Pseudomonas sp. strain Y2 involved in the conversion of styrene to phenylacetate (upper catabolic pathway) has been cloned and sequenced. Four catabolic genes, styABCD, and two regulatory genes, stySR, were identified. This gene cluster when transferred to Escherichia coli W confers to this phenylacetate-degrading host the ability to grow on styrene as the sole carbon and energy source. Genes styABCD are homologous to those encoding the styrene upper catabolic pathway in Pseudomonas fluorescens ST. Northern blot analyses have confirmed that genes styABCD constitute a transcription unit. The transcription start site of the sty operon was mapped 33 nucleotides upstream of the styA translational start codon. The styS and styR genes, which form an independent transcriptional unit, are located upstream of the styABCD operon, and their gene products show high similarity to members of the superfamily of two-component signal transduction systems. The styS gene product is homologous to histidine kinase proteins, whereas the styR gene product exhibits similarity at its N-terminal domain with cluster 1 of receiver modules and at its C terminus with the LuxR/FixJ family 3 of DNA-binding domains. Expression of the catabolic operon decreased significantly in the absence of the stySR genes and was restored when the stySR genes were provided in trans in the presence of styrene, suggesting that the stySR system behaves as a styrene-inducible positive regulator of the styABCD operon. Finally, a gene encoding a phenylacetyl-coenzyme A ligase that catalyzes the first step in the phenylacetate catabolism (styrene lower catabolic pathway) has been identified upstream of the styS gene. This activity was found to be induced in Pseudomonas sp. strain Y2 cells grown on styrene but not present in cells grown on glycerol. These results strongly suggest that the genes responsible for the complete mineralization of styrene are clustered in the chromosome of Pseudomonas sp. strain Y2.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Centro de Investigaciones Biológicas, Velázquez 144, 28006 Madrid, Spain. Phone: 34-1-5611800. Fax: 34-1-5627518. E-mail: cibg160{at}fresno.csic.es.




This article has been cited by other articles:

  • Nikodinovic-Runic, J., Flanagan, M., Hume, A. R., Cagney, G., O'Connor, K. E. (2009). Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions. Microbiology 155: 3348-3361 [Abstract] [Full Text]  
  • Busch, A., Guazzaroni, M.-E., Lacal, J., Ramos, J. L., Krell, T. (2009). The Sensor Kinase TodS Operates by a Multiple Step Phosphorelay Mechanism Involving Two Autokinase Domains. J. Biol. Chem. 284: 10353-10360 [Abstract] [Full Text]  
  • Patrauchan, M. A., Florizone, C., Eapen, S., Gomez-Gil, L., Sethuraman, B., Fukuda, M., Davies, J., Mohn, W. W., Eltis, L. D. (2008). Roles of Ring-Hydroxylating Dioxygenases in Styrene and Benzene Catabolism in Rhodococcus jostii RHA1. J. Bacteriol. 190: 37-47 [Abstract] [Full Text]  
  • Kim, S.-H., Hisano, T., Takeda, K., Iwasaki, W., Ebihara, A., Miki, K. (2007). Crystal Structure of the Oxygenase Component (HpaB) of the 4-Hydroxyphenylacetate 3-Monooxygenase from Thermus thermophilus HB8. J. Biol. Chem. 282: 33107-33117 [Abstract] [Full Text]  
  • van Hellemond, E. W., Janssen, D. B., Fraaije, M. W. (2007). Discovery of a Novel Styrene Monooxygenase Originating from the Metagenome. Appl. Environ. Microbiol. 73: 5832-5839 [Abstract] [Full Text]  
  • Busch, A., Lacal, J., Martos, A., Ramos, J. L., Krell, T. (2007). Bacterial sensor kinase TodS interacts with agonistic and antagonistic signals. Proc. Natl. Acad. Sci. USA 104: 13774-13779 [Abstract] [Full Text]  
  • del Peso-Santos, T., Bartolome-Martin, D., Fernandez, C., Alonso, S., Garcia, J. L., Diaz, E., Shingler, V., Perera, J. (2006). Coregulation by Phenylacetyl-Coenzyme A-Responsive PaaX Integrates Control of the Upper and Lower Pathways for Catabolism of Styrene by Pseudomonas sp. Strain Y2. J. Bacteriol. 188: 4812-4821 [Abstract] [Full Text]  
  • Mooney, A., O'Leary, N. D., Dobson, A. D. W. (2006). Cloning and Functional Characterization of the styE Gene, Involved in Styrene Transport in Pseudomonas putida CA-3. Appl. Environ. Microbiol. 72: 1302-1309 [Abstract] [Full Text]  
  • Leoni, L., Rampioni, G., Di Stefano, V., Zennaro, E. (2005). Dual Role of Response Regulator StyR in Styrene Catabolism Regulation. Appl. Environ. Microbiol. 71: 5411-5419 [Abstract] [Full Text]  
  • O'Leary, N. D., O'Connor, K. E., Ward, P., Goff, M., Dobson, A. D. W. (2005). Genetic Characterization of Accumulation of Polyhydroxyalkanoate from Styrene in Pseudomonas putida CA-3. Appl. Environ. Microbiol. 71: 4380-4387 [Abstract] [Full Text]  
  • Tropel, D., van der Meer, J. R. (2004). Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds. Microbiol. Mol. Biol. Rev. 68: 474-500 [Abstract] [Full Text]  
  • Otto, K., Hofstetter, K., Rothlisberger, M., Witholt, B., Schmid, A. (2004). Biochemical Characterization of StyAB from Pseudomonas sp. Strain VLB120 as a Two-Component Flavin-Diffusible Monooxygenase. J. Bacteriol. 186: 5292-5302 [Abstract] [Full Text]  
  • Takeda, H., Yamada, A., Miyauchi, K., Masai, E., Fukuda, M. (2004). Characterization of Transcriptional Regulatory Genes for Biphenyl Degradation in Rhodococcus sp. Strain RHA1. J. Bacteriol. 186: 2134-2146 [Abstract] [Full Text]  
  • Gisi, M. R., Xun, L. (2003). Characterization of Chlorophenol 4-Monooxygenase (TftD) and NADH:Flavin Adenine Dinucleotide Oxidoreductase (TftC) of Burkholderia cepacia AC1100. J. Bacteriol. 185: 2786-2792 [Abstract] [Full Text]  
  • Choi, E. N., Cho, M. C., Kim, Y., Kim, C.-K., Lee, K. (2003). Expansion of growth substrate range in Pseudomonas putida F1 by mutations in both cymR and todS, which recruit a ring-fission hydrolase CmtE and induce the tod catabolic operon, respectively. Microbiology 149: 795-805 [Abstract] [Full Text]  
  • Yoshida, T., Ayabe, Y., Yasunaga, M., Usami, Y., Habe, H., Nojiri, H., Omori, T. (2003). Genes involved in the synthesis of the exopolysaccharide methanolan by the obligate methylotroph Methylobacillus sp. strain 12S. Microbiology 149: 431-444 [Abstract] [Full Text]  
  • Ramos-Gonzalez, M.-I., Olson, M., Gatenby, A. A., Mosqueda, G., Manzanera, M., Campos, M. J., Vichez, S., Ramos, J. L. (2002). Cross-Regulation between a Novel Two-Component Signal Transduction System for Catabolism of Toluene in Pseudomonas mendocina and the TodST System from Pseudomonas putida. J. Bacteriol. 184: 7062-7067 [Abstract] [Full Text]  
  • Parales, R. E., Bruce, N. C., Schmid, A., Wackett, L. P. (2002). Biodegradation, Biotransformation, and Biocatalysis (B3). Appl. Environ. Microbiol. 68: 4699-4709 [Full Text]  
  • Johnson, G. R., Jain, R. K., Spain, J. C. (2002). Origins of the 2,4-Dinitrotoluene Pathway. J. Bacteriol. 184: 4219-4232 [Abstract] [Full Text]  
  • Diaz, E., Ferrandez, A., Prieto, M. A., Garcia, J. L. (2001). Biodegradation of Aromatic Compounds by Escherichia coli. Microbiol. Mol. Biol. Rev. 65: 523-569 [Abstract] [Full Text]  
  • Kasai, Y., Inoue, J., Harayama, S. (2001). The TOL Plasmid pWW0 xylN Gene Product from Pseudomonas putida Is Involved in m-Xylene Uptake. J. Bacteriol. 183: 6662-6666 [Abstract] [Full Text]  
  • O’Leary, N. D., O’Connor, K. E., Duetz, W., Dobson, A. D. W. (2001). Transcriptional regulation of styrene degradation in Pseudomonas putida CA-3. Microbiology 147: 973-979 [Abstract] [Full Text]  
  • García, B., Olivera, E. R., Miñambres, B., Carnicero, D., Muñiz, C., Naharro, G., Luengo, J. M. (2000). Phenylacetyl-Coenzyme A Is the True Inducer of the Phenylacetic Acid Catabolism Pathway in Pseudomonas putida U. Appl. Environ. Microbiol. 66: 4575-4578 [Abstract] [Full Text]  
  • Santos, P. M., Blatny, J. M., Di Bartolo, I., Valla, S., Zennaro, E. (2000). Physiological Analysis of the Expression of the Styrene Degradation Gene Cluster in Pseudomonas fluorescens ST. Appl. Environ. Microbiol. 66: 1305-1310 [Abstract] [Full Text]  
  • Kahng, H.-Y., Byrne, A. M., Olsen, R. H., Kukor, J. J. (2000). Characterization and Role of tbuX in Utilization of Toluene by Ralstonia pickettii PKO1. J. Bacteriol. 182: 1232-1242 [Abstract] [Full Text]  
  • Galán, B., Díaz, E., Prieto, M. A., García, J. L. (2000). Functional Analysis of the Small Component of the 4-Hydroxyphenylacetate 3-Monooxygenase of Escherichia coli W: a Prototype of a New Flavin:NAD(P)H Reductase Subfamily. J. Bacteriol. 182: 627-636 [Abstract] [Full Text]  
  • El-Said Mohamed, M. (2000). Biochemical and Molecular Characterization of Phenylacetate-Coenzyme A Ligase, an Enzyme Catalyzing the First Step in Aerobic Metabolism of Phenylacetic Acid in Azoarcus evansii. J. Bacteriol. 182: 286-294 [Abstract] [Full Text]  
  • Campos-García, J., Esteve, A., Vázquez-Duhalt, R., Ramos, J. L., Soberón-Chávez, G. (1999). The Branched-Chain Dodecylbenzene Sulfonate Degradation Pathway of Pseudomonas aeruginosa W51D Involves a Novel Route for Degradation of the Surfactant Lateral Alkyl Chain. Appl. Environ. Microbiol. 65: 3730-3734 [Abstract] [Full Text]  
  • Di Gennaro, P., Colmegna, A., Galli, E., Sello, G., Pelizzoni, F., Bestetti, G. (1999). A New Biocatalyst for Production of Optically Pure Aryl Epoxides by Styrene Monooxygenase from Pseudomonas fluorescens ST. Appl. Environ. Microbiol. 65: 2794-2797 [Abstract] [Full Text]  
  • Taylor, B. L., Zhulin, I. B. (1999). PAS Domains: Internal Sensors of Oxygen, Redox Potential, and Light. Microbiol. Mol. Biol. Rev. 63: 479-506 [Abstract] [Full Text]  
  • Ferrandez, A., Minambres, B., Garcia, B., Olivera, E. R., Luengo, J. M., Garcia, J. L., Diaz, E. (1998). Catabolism of Phenylacetic Acid in Escherichia coli. CHARACTERIZATION OF A NEW AEROBIC HYBRID PATHWAY. J. Biol. Chem. 273: 25974-25986 [Abstract] [Full Text]  
  • Panke, S., Witholt, B., Schmid, A., Wubbolts, M. G. (1998). Towards a Biocatalyst for (S)-Styrene Oxide Production: Characterization of the Styrene Degradation Pathway of Pseudomonas sp. Strain VLB120. Appl. Environ. Microbiol. 64: 2032-2043 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»