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J Bacteriol, March 1998, p. 1148-1153, Vol. 180, No. 5
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Assembly of Both the Head and Tail of Bacteriophage Mu Is Blocked in Escherichia coli groEL and groES Mutants

Régis Grimaud^1,* and Ariane Toussaint²

Laboratoire de Génétique des Procaryotes, Unité Transposition Bactérienne, Université Libre de Bruxelles, B1640 Rhode St Genèse, Belgium,² and Laboratoire de Microbiologie, Université Joseph Fourier, F38041 Grenoble Cedex 9, France¹

Received 11 November 1996/Accepted 18 December 1997

Like several other Escherichia coli bacteriophages, transposable phage Mu does not develop normally in groE hosts (M. Pato, M. Banerjee, L. Desmet, and A. Toussaint, J. Bacteriol. 169:5504-5509, 1987). We show here that lysates obtained upon induction of groE Mu lysogens contain free inactive tails and empty heads. GroEL and GroES are thus essential for the correct assembly of both Mu heads and Mu tails. Evidence is presented that groE mutations inhibit processing of the phage head protein gpH as well as the formation of a 25S complex suspected to be an early Mu head assembly intermediate.

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^* Corresponding author. Present address: Laboratory of Cell Biology, Building 37, Room 1B09, National Cancer Institute, Bethesda, MD 20892. Phone: (301) 435-1938. Fax: (301) 402-0450. E-mail: regis{at}helix.nih.gov.

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