This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sarker, M. R.
Right arrow Articles by Cornelis, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sarker, M. R.
Right arrow Articles by Cornelis, G. R.

 Previous Article  |  Next Article 

J Bacteriol, March 1998, p. 1207-1214, Vol. 180, No. 5
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Yersinia Yop Virulon: LcrV Is Required for Extrusion of the Translocators YopB and YopD

Mahfuzur R. Sarker,dagger Cécile Neyt, Isabelle Stainier, and Guy R. Cornelis*

Microbial Pathogenesis Unit, International Institute of Cellular and Molecular Pathology, and Faculté de Médecine, Université Catholique de Louvain, B-1200 Brussels, Belgium

Received 7 October 1997/Accepted 30 December 1997

LcrV, an essential piece of the Yop virulon, is encoded by the large lcrGVsycDyopBD operon. In spite of repeated efforts, the role of LcrV in the Yop virulon remains elusive. In an attempt to clarify this, we engineered a complete deletion of lcrV in the pYV plasmid of Yersinia enterocolitica E40 and characterized the phenotype of the mutant. Complementation experiments showed that the mutation was not polar with regard to yopB and yopD. Nevertheless the mutation abolished secretion of YopB and YopD, while secretion of the other Yops was unaffected or even increased. Northern blot analysis showed that transcription of yopD was not affected. YopD could be detected inside the bacteria, showing that the lack of its secretion was not due to a lack of translation or to proteolysis. This indicated that LcrV is specifically involved in the process of release of YopB and YopD. We then investigated the possible interactions between LcrV and YopB or YopD. We constructed a glutathione S-transferase-LcrV hybrid protein, and we observed that either YopB or YopD could be copurified with it. The same approach showed that LcrV also interacts with LcrG but not with the chaperone SycD. Using deletants of lcrV, we then identified a definite LcrG-binding domain in the C terminus of LcrV.

* Corresponding author. Mailing address: Microbial Pathogenesis Unit, Université Catholique de Louvain, Avenue Hippocrate, 74 UCL 74.49, B-1200 Brussels, Belgium. Phone: 32 2 764 74 49. Fax: 32 2 764 74 98. E-mail: cornelis{at}mipa.ucl.ac.be.

dagger Present address: Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261.




This article has been cited by other articles:

  • Lara-Tejero, M., Galan, J. E. (2009). Salmonella enterica Serovar Typhimurium Pathogenicity Island 1-Encoded Type III Secretion System Translocases Mediate Intimate Attachment to Nonphagocytic Cells. Infect. Immun. 77: 2635-2642 [Abstract] [Full Text]  
  • Sofer-Podesta, C., Ang, J., Hackett, N. R., Senina, S., Perlin, D., Crystal, R. G., Boyer, J. L. (2009). Adenovirus-Mediated Delivery of an Anti-V Antigen Monoclonal Antibody Protects Mice against a Lethal Yersinia pestis Challenge. Infect. Immun. 77: 1561-1568 [Abstract] [Full Text]  
  • Ivanov, M. I., Noel, B. L., Rampersaud, R., Mena, P., Benach, J. L., Bliska, J. B. (2008). Vaccination of Mice with a Yop Translocon Complex Elicits Antibodies That Are Protective against Infection with F1- Yersinia pestis. Infect. Immun. 76: 5181-5190 [Abstract] [Full Text]  
  • Riordan, K. E., Sorg, J. A., Berube, B. J., Schneewind, O. (2008). Impassable YscP Substrates and Their Impact on the Yersinia enterocolitica Type III Secretion Pathway. J. Bacteriol. 190: 6204-6216 [Abstract] [Full Text]  
  • Caroline, G., Eric, F., Bohn, Y.-S. T., Sylvie, E., Attree, I. (2008). Oligomerization of PcrV and LcrV, Protective Antigens of Pseudomonas aeruginosa and Yersinia pestis. J. Biol. Chem. 283: 23940-23949 [Abstract] [Full Text]  
  • Meyer, D., Cunnac, S., Gueneron, M., Declercq, C., Van Gijsegem, F., Lauber, E., Boucher, C., Arlat, M. (2006). PopF1 and PopF2, Two Proteins Secreted by the Type III Protein Secretion System of Ralstonia solanacearum, Are Translocators Belonging to the HrpF/NopX Family. J. Bacteriol. 188: 4903-4917 [Abstract] [Full Text]  
  • Picking, W. L., Nishioka, H., Hearn, P. D., Baxter, M. A., Harrington, A. T., Blocker, A., Picking, W. D. (2005). IpaD of Shigella flexneri Is Independently Required for Regulation of Ipa Protein Secretion and Efficient Insertion of IpaB and IpaC into Host Membranes. Infect. Immun. 73: 1432-1440 [Abstract] [Full Text]  
  • Petnicki-Ocwieja, T., van Dijk, K., Alfano, J. R. (2005). The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator. J. Bacteriol. 187: 649-663 [Abstract] [Full Text]  
  • Goure, J., Pastor, A., Faudry, E., Chabert, J., Dessen, A., Attree, I. (2004). The V Antigen of Pseudomonas aeruginosa Is Required for Assembly of the Functional PopB/PopD Translocation Pore in Host Cell Membranes. Infect. Immun. 72: 4741-4750 [Abstract] [Full Text]  
  • Olsson, J., Edqvist, P. J., Broms, J. E., Forsberg, A., Wolf-Watz, H., Francis, M. S. (2004). The YopD Translocator of Yersinia pseudotuberculosis Is a Multifunctional Protein Comprised of Discrete Domains. J. Bacteriol. 186: 4110-4123 [Abstract] [Full Text]  
  • Nogawa, H., Kuwae, A., Matsuzawa, T., Abe, A. (2004). The Type III Secreted Protein BopD in Bordetella bronchiseptica Is Complexed with BopB for Pore Formation on the Host Plasma Membrane. J. Bacteriol. 186: 3806-3813 [Abstract] [Full Text]  
  • Burr, S. E., Stuber, K., Frey, J. (2003). The ADP-Ribosylating Toxin, AexT, from Aeromonas salmonicida subsp. salmonicida Is Translocated via a Type III Secretion Pathway. J. Bacteriol. 185: 6583-6591 [Abstract] [Full Text]  
  • Broms, J. E., Forslund, A.-L., Forsberg, A., Francis, M. S. (2003). Dissection of homologous translocon operons reveals a distinct role for YopD in type III secretion by Yersinia pseudotuberculosis. Microbiology 149: 2615-2626 [Abstract] [Full Text]  
  • Brubaker, R. R. (2003). Interleukin-10 and Inhibition of Innate Immunity to Yersiniae: Roles of Yops and LcrV (V Antigen). Infect. Immun. 71: 3673-3681 [Full Text]  
  • Allmond, L. R., Karaca, T. J., Nguyen, V. N., Nguyen, T., Wiener-Kronish, J. P., Sawa, T. (2003). Protein Binding between PcrG-PcrV and PcrH-PopB/PopD Encoded by the pcrGVH-popBD Operon of the Pseudomonas aeruginosa Type III Secretion System. Infect. Immun. 71: 2230-2233 [Abstract] [Full Text]  
  • Foultier, B., Troisfontaines, P., Vertommen, D., Marenne, M.-N., Rider, M., Parsot, C., Cornelis, G. R. (2003). Identification of Substrates and Chaperone from the Yersinia enterocolitica 1B Ysa Type III Secretion System. Infect. Immun. 71: 242-253 [Abstract] [Full Text]  
  • Lawton, D. G., Longstaff, C., Wallace, B. A., Hill, J., Leary, S. E. C., Titball, R. W., Brown, K. A. (2002). Interactions of the Type III Secretion Pathway Proteins LcrV and LcrG from Yersinia pestis Are Mediated by Coiled-Coil Domains. J. Biol. Chem. 277: 38714-38722 [Abstract] [Full Text]  
  • Grosdent, N., Maridonneau-Parini, I., Sory, M.-P., Cornelis, G. R. (2002). Role of Yops and Adhesins in Resistance of Yersinia enterocolitica to Phagocytosis. Infect. Immun. 70: 4165-4176 [Abstract] [Full Text]  
  • Sauvonnet, N., Pradet-Balade, B., Garcia-Sanz, J. A., Cornelis, G. R. (2002). Regulation of mRNA Expression in Macrophages after Yersinia enterocolitica Infection. ROLE OF DIFFERENT Yop EFFECTORS. J. Biol. Chem. 277: 25133-25142 [Abstract] [Full Text]  
  • Denecker, G., Totemeyer, S., Mota, L. J., Troisfontaines, P., Lambermont, I., Youta, C., Stainier, I., Ackermann, M., Cornelis, G. R. (2002). Effect of Low- and High-Virulence Yersinia enterocolitica Strains on the Inflammatory Response of Human Umbilical Vein Endothelial Cells. Infect. Immun. 70: 3510-3520 [Abstract] [Full Text]  
  • Bleves, S., Marenne, M.-N., Detry, G., Cornelis, G. R. (2002). Up-Regulation of the Yersinia enterocolitica yop Regulon by Deletion of the Flagellum Master Operon flhDC. J. Bacteriol. 184: 3214-3223 [Abstract] [Full Text]  
  • Trcek, J., Wilharm, G., Jacobi, C. A., Heesemann, J. (2002). Yersinia enterocolitica YopQ: strain-dependent cytosolic accumulation and post-translational secretion. Microbiology 148: 1457-1465 [Abstract] [Full Text]  
  • Sing, A., Roggenkamp, A., Geiger, A. M., Heesemann, J. (2002). Yersiniaenterocolitica Evasion of the Host Innate Immune Response by V Antigen-Induced IL-10 Production of Macrophages Is Abrogated in IL-10-Deficient Mice. J. Immunol. 168: 1315-1321 [Abstract] [Full Text]  
  • Shime, N., Sawa, T., Fujimoto, J., Faure, K., Allmond, L. R., Karaca, T., Swanson, B. L., Spack, E. G., Wiener-Kronish, J. P. (2001). Therapeutic Administration of Anti-PcrV F(ab')2 in Sepsis Associated with Pseudomonas aeruginosa. J. Immunol. 167: 5880-5886 [Abstract] [Full Text]  
  • DeBord, K. L., Lee, V. T., Schneewind, O. (2001). Roles of LcrG and LcrV during Type III Targeting of Effector Yops by Yersinia enterocolitica. J. Bacteriol. 183: 4588-4598 [Abstract] [Full Text]  
  • Boyd, A. P., Lambermont, I., Cornelis, G. R. (2000). Competition between the Yops of Yersinia enterocolitica for Delivery into Eukaryotic Cells: Role of the SycE Chaperone Binding Domain of YopE. J. Bacteriol. 182: 4811-4821 [Abstract] [Full Text]  
  • Cornelis, G. R. (2000). Molecular and cell biology aspects of plague. Proc. Natl. Acad. Sci. USA 97: 8778-8783 [Abstract] [Full Text]  
  • Hein, J., Kempf, V. A. J., Diebold, J., Bucheler, N., Preger, S., Horak, I., Sing, A., Kramer, U., Autenrieth, I. B. (2000). Interferon Consensus Sequence Binding Protein Confers Resistance against Yersinia enterocolitica. Infect. Immun. 68: 1408-1417 [Abstract] [Full Text]  
  • Fields, K. A., Nilles, M. L., Cowan, C., Straley, S. C. (1999). Virulence Role of V Antigen of Yersinia pestis at the Bacterial Surface. Infect. Immun. 67: 5395-5408 [Abstract] [Full Text]  
  • Fields, K. A., Straley, S. C. (1999). LcrV of Yersinia pestis Enters Infected Eukaryotic Cells by a Virulence Plasmid-Independent Mechanism. Infect. Immun. 67: 4801-4813 [Abstract] [Full Text]  
  • Cornelis, G. R., Boland, A., Boyd, A. P., Geuijen, C., Iriarte, M., Neyt, C., Sory, M.-P., Stainier, I. (1998). The Virulence Plasmid of Yersinia, an Antihost Genome. Microbiol. Mol. Biol. Rev. 62: 1315-1352 [Abstract] [Full Text]  
  • Cornelis, G. R. (1998). The Yersinia Deadly Kiss. J. Bacteriol. 180: 5495-5504 [Full Text]  
  • Jackson, M. W., Day, J. B., Plano, G. V. (1998). YscB of Yersinia pestis Functions as a Specific Chaperone for YopN. J. Bacteriol. 180: 4912-4921 [Abstract] [Full Text]  
  • Nilles, M. L., Fields, K. A., Straley, S. C. (1998). The V Antigen of Yersinia pestis Regulates Yop Vectorial Targeting as Well as Yop Secretion through Effects on YopB and LcrG. J. Bacteriol. 180: 3410-3420 [Abstract] [Full Text]  
  • Sarker, M. R., Sory, M.-P., Boyd, A. P., Iriarte, M., Cornelis, G. R. (1998). LcrG is Required for Efficient Translocation of Yersinia Yop Effector Proteins into Eukaryotic Cells. Infect. Immun. 66: 2976-2979 [Abstract] [Full Text]  
  • Boland, A., Cornelis, G. R. (1998). Role of YopP in Suppression of Tumor Necrosis Factor Alpha Release by Macrophages during Yersinia Infection. Infect. Immun. 66: 1878-1884 [Abstract] [Full Text]  
  • Lee, V. T., Tam, C., Schneewind, O. (2000). LcrV, a Substrate for Yersinia enterocolitica Type III Secretion, Is Required for Toxin Targeting into the Cytosol of HeLa Cells. J. Biol. Chem. 275: 36869-36875 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»