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J Bacteriol, March 1998, p. 1334-1337, Vol. 180, No. 5
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Purification of an Extracellular Signaling Molecule Involved in Production of Aerial Mycelium by Streptomyces coelicolor

Justin R. Nodwell and Richard Losick*

Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138

Received 17 October 1997/Accepted 24 December 1997

We have extensively purified a factor from conditioned medium that restores aerial mycelium formation to a mutant of Streptomyces coelicolor that is defective in morphological differentiation. Response to this factor is shown to depend on the presence of the BldK oligopeptide import system. We suggest that this substance acts at the first step in a putative cascade of developmental regulatory signals.

* Corresponding author. Mailing address: Department of Molecular and Cellular Biology, Biological Laboratories, 16 Divinity Ave., Harvard University, Cambridge, MA 02138. Phone: (617) 495-1774. Fax: (617) 496-4642. E-mail: losick{at}biosun.harvard.edu.




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