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 Previous Article

J Bacteriol, March 1998, p. 1342-1345, Vol. 180, No. 5
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of Mutants Devoid of Neutral Trehalase Activity in the Fission Yeast Schizosaccharomyces pombe: Partial Protection from Heat Shock and High-Salt Stress

Jose Cansado, Teresa Soto, Juana Fernandez, Jero Vicente-Soler, and Mariano Gacto*

Department of Genetics and Microbiology, Facultad de Biologia, University of Murcia, 30071 Murcia, Spain

Received 29 October 1997/Accepted 29 December 1997

Exposure of cells of Schizosaccharomyces pombe to heat shock or osmotic upshift results in an increased level of neutral trehalase activity, which is responsible for hydrolysis of intracellular trehalose. We constructed S. pombe mutants lacking neutral trehalase activity by gene replacement at the newly defined ntp1+ locus. Analysis of these mutants revealed that a twofold increase in trehalose accumulation, enhanced acquired thermoresistance, and marked salt tolerance characterized their ability to grow in liquid and solid media. Analysis of the expression of the trehalase gene under heat shock and osmotic upshift revealed the transcriptional activation of ntp1+ in response to both stresses.

* Corresponding author. Mailing address: Department of Genetics and Microbiology, University of Murcia, 30071 Murcia, Spain. Phone: (34) 68 307100. Fax: (34) 68 363963. E-mail: maga{at}fcu.um.es.




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