This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zeilstra-Ryalls, J. H.
Right arrow Articles by Kaplan, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zeilstra-Ryalls, J. H.
Right arrow Articles by Kaplan, S.

 Previous Article  |  Next Article 

J Bacteriol, March 1998, p. 1496-1503, Vol. 180, No. 6
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Role of the fnrL Gene in Photosystem Gene Expression and Photosynthetic Growth of Rhodobacter sphaeroides 2.4.1

Jill H. Zeilstra-Ryallsdagger and Samuel Kaplan*

Department of Microbiology and Molecular Genetics, University of Texas Health Science Center---Houston, Houston, Texas 77030

Received 21 August 1997/Accepted 3 January 1998

Anoxygenic photosynthetic growth of Rhodobacter sphaeroides 2.4.1 requires a functional fnrL gene, which encodes the anaerobic regulator, FnrL. Using transcriptional fusions to the puc operon in which the upstream FNR consensus-like sequence is either present or absent, we obtained results that suggest that FnrL has both a direct and an indirect role in puc operon expression. In addition to FnrL, several other factors, including the two-component Prr regulatory system and the transcriptional repressor PpsR, are known to mediate oxygen control of photosynthesis gene expression in this organism. Therefore, we examined the relationship between FnrL and these other regulatory elements. Our results indicate that while mutations of prr or ppsR can lead to an increase in expression of some photosynthesis genes under aerobic and anaerobic conditions, regardless of the presence or absence of FnrL, there remains an absolute requirement for a functional fnrL gene for photosynthetic growth. We examined the potential role(s) of FnrL in photosynthetic growth by considering several target genes which may be required for this growth mode.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, University of Texas Health Science Center---Houston, 6431 Fannin St., Houston, TX 77030. Phone: (713) 500-5502. Fax: (713) 500-5499. E-mail: skaplan{at}utmmg.med.uth.tmc.edu.

dagger Permanent address: Department of Biological Sciences, Oakland University, Rochester, MI 48309.




This article has been cited by other articles:

  • Eraso, J. M., Kaplan, S. (2009). Regulation of Gene Expression by PrrA in Rhodobacter sphaeroides 2.4.1: Role of Polyamines and DNA Topology. J. Bacteriol. 191: 4341-4352 [Abstract] [Full Text]  
  • Eraso, J. M., Roh, J. H., Zeng, X., Callister, S. J., Lipton, M. S., Kaplan, S. (2008). Role of the Global Transcriptional Regulator PrrA in Rhodobacter sphaeroides 2.4.1: Combined Transcriptome and Proteome Analysis. J. Bacteriol. 190: 4831-4848 [Abstract] [Full Text]  
  • Ouchane, S., Picaud, M., Therizols, P., Reiss-Husson, F., Astier, C. (2007). Global Regulation of Photosynthesis and Respiration by FnrL: THE FIRST TWO TARGETS IN THE TETRAPYRROLE PATHWAY. J. Biol. Chem. 282: 7690-7699 [Abstract] [Full Text]  
  • Zeilstra-Ryalls, J. H., Schornberg, K. L. (2006). Analysis of hemF Gene Function and Expression in Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 188: 801-804 [Abstract] [Full Text]  
  • Zeller, T., Moskvin, O. V., Li, K., Klug, G., Gomelsky, M. (2005). Transcriptome and Physiological Responses to Hydrogen Peroxide of the Facultatively Phototrophic Bacterium Rhodobacter sphaeroides. J. Bacteriol. 187: 7232-7242 [Abstract] [Full Text]  
  • Mao, L., Mackenzie, C., Roh, J. H., Eraso, J. M., Kaplan, S., Resat, H. (2005). Combining microarray and genomic data to predict DNA binding motifs. Microbiology 151: 3197-3213 [Abstract] [Full Text]  
  • Roh, J. H., Smith, W. E., Kaplan, S. (2004). Effects of Oxygen and Light Intensity on Transcriptome Expression in Rhodobacter sphaeroides 2.4.1: REDOX ACTIVE GENE EXPRESSION PROFILE. J. Biol. Chem. 279: 9146-9155 [Abstract] [Full Text]  
  • Cho, S.-H., Youn, S.-H., Lee, S.-R., Yim, H.-S., Kang, S.-O. (2004). Redox property and regulation of PpsR, a transcriptional repressor of photosystem gene expression in Rhodobacter sphaeroides. Microbiology 150: 697-706 [Abstract] [Full Text]  
  • Gomelsky, L., Sram, J., Moskvin, O. V., Horne, I. M., Dodd, H. N., Pemberton, J. M., McEwan, A. G., Kaplan, S., Gomelsky, M. (2003). Identification and in vivo characterization of PpaA, a regulator of photosystem formation in Rhodobacter sphaeroides. Microbiology 149: 377-388 [Abstract] [Full Text]  
  • Roh, J. H., Kaplan, S. (2002). Interdependent Expression of the ccoNOQP-rdxBHIS Loci in Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 184: 5330-5338 [Abstract] [Full Text]  
  • Wood, N. J., Alizadeh, T., Bennett, S., Pearce, J., Ferguson, S. J., Richardson, D. J., Moir, J. W. B. (2001). Maximal Expression of Membrane-Bound Nitrate Reductase in Paracoccus Is Induced by Nitrate via a Third FNR-Like Regulator Named NarR. J. Bacteriol. 183: 3606-3613 [Abstract] [Full Text]  
  • Roh, J. H., Kaplan, S. (2000). Genetic and Phenotypic Analyses of the rdx Locus of Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 182: 3475-3481 [Abstract] [Full Text]  
  • Oh, J.-I., Eraso, J. M., Kaplan, S. (2000). Interacting Regulatory Circuits Involved in Orderly Control of Photosynthesis Gene Expression in Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 182: 3081-3087 [Abstract] [Full Text]  
  • Pasternak, C., Haberzettl, K., Klug, G. (1999). Thioredoxin Is Involved in Oxygen-Regulated Formation of the Photosynthetic Apparatus of Rhodobacter sphaeroides. J. Bacteriol. 181: 100-106 [Abstract] [Full Text]  
  • Zeilstra-Ryalls, J., Gomelsky, M., Eraso, J. M., Yeliseev, A., O'Gara, J., Kaplan, S. (1998). Control of Photosystem Formation in Rhodobacter sphaeroides. J. Bacteriol. 180: 2801-2809 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»