This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Steffen, P.
Right arrow Articles by Ullmann, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steffen, P.
Right arrow Articles by Ullmann, A.

 Previous Article  |  Next Article 

J Bacteriol, March 1998, p. 1567-1569, Vol. 180, No. 6
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Hybrid Bordetella pertussis-Escherichia coli RNA Polymerases: Selectivity of Promoter Activation

Pierre Steffen and Agnes Ullmann*

Unité de Biochimie Cellulaire, Institut Pasteur, 75724 Paris Cedex 15, France

Received 24 October 1997/Accepted 9 January 1998

We constructed hybrid Bordetella pertussis-Escherichia coli RNA polymerases and compared productive interactions between transcription activators and cognate RNA polymerase subunits in an in vitro transcription system. Virulence-associated genes of B. pertussis, in the presence of their activator BvgA, are transcribed by all variants of hybrid RNA polymerases, whereas transcription at the E. coli lac promoter regulated by the cyclic AMP-catabolite gene activator protein has an absolute requirement for the E. coli alpha  subunit. This suggests that activator contact sites involve a high degree of selectivity.

* Corresponding author. Mailing address: Unité de Biochimie Cellulaire, 28 rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: 33 (1) 45 68 83 85. Fax: 33 (1) 40 61 30 19. E-mail: ullmann{at}pasteur.fr.




This article has been cited by other articles:

  • Richard, C. L., Tandon, A., Sloan, N. R., Kranz, R. G. (2003). RNA Polymerase Subunit Requirements for Activation by the Enhancer-binding Protein Rhodobacter capsulatus NtrC. J. Biol. Chem. 278: 31701-31708 [Abstract] [Full Text]  
  • Peck, M. C., Gaal, T., Fisher, R. F., Gourse, R. L., Long, S. R. (2002). The RNA Polymerase {alpha} Subunit from Sinorhizobium meliloti Can Assemble with RNA Polymerase Subunits from Escherichia coli and Function in Basal and Activated Transcription both In Vivo and In Vitro. J. Bacteriol. 184: 3808-3814 [Abstract] [Full Text]  
  • Lohrke, S. M., Yang, H., Jin, S. (2001). Reconstitution of Acetosyringone-Mediated Agrobacterium tumefaciens Virulence Gene Expression in the Heterologous Host Escherichia coli. J. Bacteriol. 183: 3704-3711 [Abstract] [Full Text]  
  • Lohrke, S. M., Nechaev, S., Yang, H., Severinov, K., Jin, S. J. (1999). Transcriptional Activation of Agrobacterium tumefaciens Virulence Gene Promoters in Escherichia coli Requires the A. tumefaciens rpoA Gene, Encoding the Alpha Subunit of RNA Polymerase. J. Bacteriol. 181: 4533-4539 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»