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J Bacteriol, March 1998, p. 1600-1602, Vol. 180, No. 6
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Sensory Rhodopsin II Transducer HtrII Is Also Responsible for Serine Chemotaxis in the Archaeon Halobacterium salinarum

Shaobin Hou, Alexei Brooun, Hyung Suk Yu, Tracey Freitas, and Maqsudul Alam^*

Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822

Received 31 October 1997/Accepted 30 December 1997

Previously, we demonstrated that the methyl-accepting protein HtrII is the transducer for photoreceptor sensory rhodopsin II. Here, we provide experimental evidence that HtrII is also a chemotransducer. Using an agarose-in-plug bridge method, we show that an HtrII overexpression strain has a quicker response to serine than does an HtrII deletion strain. Furthermore, an in vivo flow assay demonstrates that the deletion strain is unable to modulate methylesterase activity after serine addition or photostimulation, while the overexpression strain shows distinct methanol peaks following both types of stimuli.

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^* Corresponding author. Mailing address: Department of Microbiology, 2538 The Mall, Snyder Hall 207, University of Hawaii, Honolulu, HI 96822. Phone: (808) 956-8553. Fax: (808) 956-5339. E-mail: alam{at}hawaii.edu.

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