This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nilsen, T.
Right arrow Articles by Holo, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nilsen, T.
Right arrow Articles by Holo, H.

 Previous Article  |  Next Article 

J Bacteriol, April 1998, p. 1848-1854, Vol. 180, No. 7
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

An Exported Inducer Peptide Regulates Bacteriocin Production in Enterococcus faecium CTC492

Trine Nilsen, Ingolf F. Nes, and Helge Holo*

Laboratory of Microbial Gene Technology, Agricultural University of Norway, N-1432 Ås, Norway

Received 30 June 1997/Accepted 2 February 1998

Production of the bacteriocins enterocin A and enterocin B in Enterococcus faecium CTC492 was dependent on the presence of an extracellular peptide produced by the strain itself. This induction factor (EntF) was purified, and amino acid sequencing combined with DNA sequencing of the corresponding gene identified it as a peptide of 25 amino acids. The gene encodes a prepeptide of 41 amino acids, including a 16-amino-acid leader peptide of the double-glycine type. Environmental factors influenced the level of bacteriocin production in E. faecium CTC492. The optimal pH for bacteriocin production was 6.2. At pH 5.5, growth was slow, and very little bacteriocin was formed. The presence of NaCl or ethanol (EtOH) was also inhibitory to bacteriocin production, and at high concentrations of these solutes, no bacteriocin production was observed. The induction factor induced its own synthesis, and by dilution of the culture 106 times or more, nonproducing cultures were obtained. Bacteriocin production was induced in these cultures by addition of EntF. The response was linear, and low bacteriocin production could be induced by about 10-17 M EntF. This response was attenuated by low pH or the presence of high concentrations of NaCl or EtOH, and 300 times more EntF was needed to induce detectable bacteriocin production in the presence of 6.5% NaCl. High levels of bacteriocin production in cultures grown at low pH or in the presence of high concentrations of NaCl or EtOH were obtained by addition of sufficient amounts of EntF.

* Corresponding author. Mailing address: Laboratory of Microbial Gene Technology, Agricultural University of Norway, P.O. Box 5051, N-1432 Ås, Norway. Phone: 47 64 94 94 68. Fax: 47 64 94 14 65. E-mail: helge.holo{at}ibf.nlh.no.




This article has been cited by other articles:

  • van Belkum, M. J., Derksen, D. J., Franz, C. M. A. P., Vederas, J. C. (2007). Structure function relationship of inducer peptide pheromones involved in bacteriocin production in Carnobacterium maltaromaticum and Enterococcus faecium. Microbiology 153: 3660-3666 [Abstract] [Full Text]  
  • Nes, I. F., Diep, D. B., Holo, H. (2007). Bacteriocin Diversity in Streptococcus and Enterococcus. J. Bacteriol. 189: 1189-1198 [Full Text]  
  • Verluyten, J., Messens, W., De Vuyst, L. (2004). Sodium Chloride Reduces Production of Curvacin A, a Bacteriocin Produced by Lactobacillus curvatus Strain LTH 1174, Originating from Fermented Sausage. Appl. Environ. Microbiol. 70: 2271-2278 [Abstract] [Full Text]  
  • Maldonado, A., Jimenez-Diaz, R., Ruiz-Barba, J. L. (2004). Induction of Plantaricin Production in Lactobacillus plantarum NC8 after Coculture with Specific Gram-Positive Bacteria Is Mediated by an Autoinduction Mechanism. J. Bacteriol. 186: 1556-1564 [Abstract] [Full Text]  
  • Johnsborg, O., Diep, D. B., Nes, I. F. (2003). Structural Analysis of the Peptide Pheromone Receptor PlnB, a Histidine Protein Kinase from Lactobacillus plantarum. J. Bacteriol. 185: 6913-6920 [Abstract] [Full Text]  
  • Nilsen, T., Nes, I. F., Holo, H. (2003). Enterolysin A, a Cell Wall-Degrading Bacteriocin from Enterococcus faecalis LMG 2333. Appl. Environ. Microbiol. 69: 2975-2984 [Abstract] [Full Text]  
  • Leal-Sanchez, M. V., Jimenez-Diaz, R., Maldonado-Barragan, A., Garrido-Fernandez, A., Ruiz-Barba, J. L. (2002). Optimization of Bacteriocin Production by Batch Fermentation of Lactobacillus plantarum LPCO10. Appl. Environ. Microbiol. 68: 4465-4471 [Abstract] [Full Text]  
  • Li, H., O'Sullivan, D. J. (2002). Heterologous Expression of the Lactococcus lactis Bacteriocin, Nisin, in a Dairy Enterococcus Strain. Appl. Environ. Microbiol. 68: 3392-3400 [Abstract] [Full Text]  
  • Nilsson, L., Nielsen, M. K., Ng, Y., Gram, L. (2002). Role of Acetate in Production of an Autoinducible Class IIa Bacteriocin in Carnobacterium piscicola A9b. Appl. Environ. Microbiol. 68: 2251-2260 [Abstract] [Full Text]  
  • Uteng, M., Hauge, H. H., Brondz, I., Nissen-Meyer, J., Fimland, G. (2002). Rapid Two-Step Procedure for Large-Scale Purification of Pediocin-Like Bacteriocins and Other Cationic Antimicrobial Peptides from Complex Culture Medium. Appl. Environ. Microbiol. 68: 952-956 [Abstract] [Full Text]  
  • Leroy, F., De Vuyst, L. (2001). Growth of the Bacteriocin-Producing Lactobacillus sakei Strain CTC 494 in MRS Broth Is Strongly Reduced Due to Nutrient Exhaustion: a Nutrient Depletion Model for the Growth of Lactic Acid Bacteria. Appl. Environ. Microbiol. 67: 4407-4413 [Abstract] [Full Text]  
  • Cintas, L. M., Casaus, M. P., Herranz, C., Nes, I. F., Hernandez, P. E. (2001). Review: Bacteriocins of Lactic Acid Bacteria. Food Science and Technology International 7: 281-305 [Abstract]  
  • Magnusson, J., Schnürer, J. (2001). Lactobacillus coryniformis subsp. coryniformis Strain Si3 Produces a Broad-Spectrum Proteinaceous Antifungal Compound. Appl. Environ. Microbiol. 67: 1-5 [Abstract] [Full Text]  
  • Franz, C. M. A. P., van Belkum, M. J., Worobo, R. W., Vederas, J. C., Stiles, M. E. (2000). Characterization of the genetic locus responsible for production and immunity of carnobacteriocin A: the immunity gene confers cross-protection to enterocin B. Microbiology 146: 621-631 [Abstract] [Full Text]  
  • Leroy, F., de Vuyst, L. (1999). The Presence of Salt and a Curing Agent Reduces Bacteriocin Production by Lactobacillus sakei CTC 494, a Potential Starter Culture for Sausage Fermentation. Appl. Environ. Microbiol. 65: 5350-5356 [Abstract] [Full Text]  
  • Callewaert, R., Holo, H., Devreese, B., Van Beeumen, J., Nes, I., De Vuyst, L. (1999). Characterization and production of amylovorin L471, a bacteriocin purified from Lactobacillus amylovorus DCE 471 by a novel three-step method. Microbiology 145: 2559-2568 [Abstract] [Full Text]  
  • Franz, C. M. A. P., Worobo, R. W., Quadri, L. E. N., Schillinger, U., Holzapfel, W. H., Vederas, J. C., Stiles, M. E. (1999). Atypical Genetic Locus Associated with Constitutive Production of Enterocin B by Enterococcus faecium BFE 900. Appl. Environ. Microbiol. 65: 2170-2178 [Abstract] [Full Text]  
  • Rose, N. L., Sporns, P., McMullen, L. M. (1999). Detection of Bacteriocins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Appl. Environ. Microbiol. 65: 2238-2242 [Abstract] [Full Text]  
  • O'Keeffe, T., Hill, C., Ross, R. P. (1999). Characterization and Heterologous Expression of the Genes Encoding Enterocin A Production, Immunity, and Regulation in Enterococcus faecium DPC1146. Appl. Environ. Microbiol. 65: 1506-1515 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»