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J Bacteriol, April 1998, p. 1965-1969, Vol. 180, No. 7
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evidence for a Regulatory Link of Nitrogen Fixation and Photosynthesis in Rhodobacter capsulatus via HvrA

Monika Kern,dagger Paul-Bertram Kamp, Annette Paschen, Bernd Masepohl, and Werner Klipp*

Ruhr-Universität Bochum, Fakultät für Biologie, Lehrstuhl für Biologie der Mikroorganismen, D-44780 Bochum, Germany

Received 14 October 1997/Accepted 27 January 1998

A Rhodobacter capsulatus reporter strain, carrying a constitutively expressed nifA gene and a nifH-lacZ gene fusion, was used for random transposon Tn5 mutagenesis to search for genes required for the NtrC-independent ammonium repression of NifA activity. A mutation in hvrA, which is known to be involved in low-light activation of the photosynthetic apparatus, released both ammonium and oxygen control of nifH expression in this reporter strain, demonstrating a regulatory link of nitrogen fixation and photosynthesis via HvrA. In addition, a significant increase in bacteriochlorophyll a (BChla) content was found in cells under nitrogen-fixing conditions. HvrA was not involved in this up-regulation of BChla. Instead, the presence of active nitrogenase seemed to be sufficient for this process, since no increase in BChla content was observed in different nif mutants.

* Corresponding author. Mailing address: Ruhr-Universität Bochum, Fakultät für Biologie, Lehrstuhl für Biologie der Mikroorganismen, D-44780 Bochum, Germany. Phone: 49-(0)234-700-3100. Fax: 49-(0)234-7094-620. E-mail: werner.klipp{at}ruhr-uni-bochum.de.

dagger Present address: Klinische Kooperationseinheit für Dermato-Onkologie am Klinikum Mannheim, Theodor-Kutzerufer 1, D-68135 Mannheim, Germany.




This article has been cited by other articles:

  • Masuda, S., Bauer, C. E. (2004). Null Mutation of HvrA Compensates for Loss of an Essential relA/spoT-Like Gene in Rhodobacter capsulatus. J. Bacteriol. 186: 235-239 [Abstract] [Full Text]  
  • Tichi, M. A., Tabita, F. R. (2001). Interactive Control of Rhodobacter capsulatus Redox-Balancing Systems during Phototrophic Metabolism. J. Bacteriol. 183: 6344-6354 [Abstract] [Full Text]  


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