This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamashino, T.
Right arrow Articles by Mizuno, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamashino, T.
Right arrow Articles by Mizuno, T.

 Previous Article  |  Next Article 

J Bacteriol, April 1998, p. 2257-2261, Vol. 180, No. 8
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The yhhP Gene Encoding a Small Ubiquitous Protein Is Fundamental for Normal Cell Growth of Escherichia coli

Takafumi Yamashino, Mototoshi Isomura, Chiharu Ueguchi, and Takeshi Mizuno*

Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-1806, Japan

Received 18 December 1997/Accepted 20 February 1998

H-NS is a major constituent of the Escherichia coli nucleoid, whereas sigma S is a stress-induced sigma factor. An hns null mutation affects the cellular content of sigma S in such a way that a remarkable accumulation of sigma S is observed in the logarithmic growth phase, which results in enhanced expression of a number of sigma S-dependent genes, including the katE gene. We isolated an extragenic mutation that affects the expression of the katE-lacZ fusion gene in the Delta hns background. The relevant gene was identified as yhhP, which encodes a small polypeptide of 81 amino acids. Lesion of this gene seemed to affect the stability of sigma S. A deletion analysis of yhhP revealed that this small protein plays a fundamental role in the general physiology of E. coli. The yhhP-deficient cell is not capable of growing in standard laboratory rich medium (i.e., Luria broth), resulting in the formation of filamentous cells. Homologs of this intriguing protein occur in a wide variety of bacterial species, including archaeal species.

* Corresponding author. Mailing address: Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-1806, Japan. Phone: (81)-52-789-4089. Fax: (81)-52-789-4091. E-mail: i45455a{at}nucc.cc.nagoya-u.ac.jp.




This article has been cited by other articles:

  • Navarre, W. W., McClelland, M., Libby, S. J., Fang, F. C. (2007). Silencing of xenogeneic DNA by H-NS--facilitation of lateral gene transfer in bacteria by a defense system that recognizes foreign DNA. Genes Dev. 21: 1456-1471 [Abstract] [Full Text]  
  • Zhou, Y., Gottesman, S. (2006). Modes of Regulation of RpoS by H-NS.. J. Bacteriol. 188: 7022-7025 [Abstract] [Full Text]  
  • Hagiwara, D., Sugiura, M., Oshima, T., Mori, H., Aiba, H., Yamashino, T., Mizuno, T. (2003). Genome-Wide Analyses Revealing a Signaling Network of the RcsC-YojN-RcsB Phosphorelay System in Escherichia coli. J. Bacteriol. 185: 5735-5746 [Abstract] [Full Text]  
  • Chou, C.-C., Lin, T.-W., Chen, C.-Y., Wang, A. H.-J. (2003). Crystal Structure of the Hyperthermophilic Archaeal DNA-Binding Protein Sso10b2 at a Resolution of 1.85 Angstroms. J. Bacteriol. 185: 4066-4073 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»