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J Bacteriol, May 1998, p. 2515-2521, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Developmental Regulation of Transcription of whiE, a Locus Specifying the Polyketide Spore Pigment in Streptomyces coelicolor A3(2)

Gabriella H. Kelemen,* Paul Brian,dagger Klas Flärdh, Leony Chamberlin, Keith F. Chater, and Mark J. Buttner

John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom

Received 31 October 1997/Accepted 23 February 1998

whiE is a complex locus that specifies the polyketide spore pigment in Streptomyces coelicolor A3(2). Two divergently oriented promoters, whiEP1 and whiEP2, were identified in the whiE gene cluster, and their activities were analyzed during colony development in wild-type and sporulation-deficient strains. Both promoters were developmentally regulated; whiEP1 and whiEP2 transcripts were detected transiently at approximately the time when sporulation septa were observed in the aerial hyphae, and transcription from both promoters depended on each of the six known "early" whi genes required for sporulation septum formation (whiA, -B, -G, -H, -I, and -J). Mutation of the late sporulation-specific sigma factor gene, sigF, had no effect on the activity of whiEP1 but blocked transcription from whiEP2. However, sigma F-containing holoenzyme was not sufficient to direct transcription of whiEP2 in vitro. The whiEP2 promoter controls expression of whiE ORFVIII, encoding a putative flavin adenine dinucleotide-dependent hydroxylase that catalyzes a late tailoring step in the spore pigment biosynthetic pathway. Disruption of whiE ORFVIII causes a change in spore color, from grey to greenish (T.-W. Yu and D. A. Hopwood, Microbiology 141:2779-2791, 1995). Consistent with these observations, construction of a sigF null mutant of S. coelicolor M145 caused the same change in spore color, showing that disruption of sigF in S. coelicolor changes the nature of the spore pigment rather than preventing its synthesis altogether.

* Corresponding author. Mailing address: John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom. Phone: (44) 1603 452571. Fax: (44) 1603 456844. E-mail: KELEMEN{at}BBSRC.AC.UK.

dagger Present address: Cromaxome Corporation, San Diego, CA 92121.

J Bacteriol, May 1998, p. 2515-2521, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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