This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishikawa, S.
Right arrow Articles by Sekiguchi, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishikawa, S.
Right arrow Articles by Sekiguchi, J.

 Previous Article  |  Next Article 

J Bacteriol, May 1998, p. 2549-2555, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Regulation of a New Cell Wall Hydrolase Gene, cwlF, Which Affects Cell Separation in Bacillus subtilis

Shu Ishikawa, Yoshiko Hara, Ryo Ohnishi, and Junichi Sekiguchi*

Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386, Japan

Received 29 September 1997/Accepted 15 February 1998

Bacillus subtilis produces a 35-kDa cell wall hydrolase, CwlF, during vegetative growth. The CwlF protein was extracted from B. subtilis cwlB sigD mutant cells and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino acid sequencing revealed that its sequence is completely identical to that of the internal region of the papQ gene product. Disruption of the papQ gene in the B. subtilis chromosome led to the complete loss of CwlF, indicating that papQ is identical to cwlF. CwlF exhibits high sequence similarity to the p60 proteins of Listeria species, NlpC proteins of Escherichia coli and Haemophilus influenzae, and Enp2 protein of Bacillus sphaericus. The beta -galactosidase activity of the cwlF-lacZ transcriptional fusion and Northern blot analysis of the cwlF gene indicated that the gene is expressed as a monocistronic operon during the exponential growth phase, and primer extension analysis suggested that the cwlF gene is transcribed mainly by Esigma A RNA polymerase and weakly by Esigma H RNA polymerase. While the cells of the cwlF-deficient mutant were about twice as long as those of the wild-type strain, the cwlF sigD double mutant cells exhibited extraordinary microfiber formation, in contrast to the filamentation of the sigD mutant. The CwlF production was not affected by the pleiotropic mutations flaD1 and degU32(Hy), which endow cells with the ability of extensive filamentation.

* Corresponding author. Mailing address: Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386, Japan. Phone: 81-268-21-5344. Fax: 81-268-21-5331. E-mail: jsekigu{at}giptc.shinshu-u.ac.jp.

J Bacteriol, May 1998, p. 2549-2555, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Uehara, T., Dinh, T., Bernhardt, T. G. (2009). LytM-Domain Factors Are Required for Daughter Cell Separation and Rapid Ampicillin-Induced Lysis in Escherichia coli. J. Bacteriol. 191: 5094-5107 [Abstract] [Full Text]  
  • Andre, G., Leenhouts, K., Hols, P., Dufrene, Y. F. (2008). Detection and Localization of Single LysM-Peptidoglycan Interactions. J. Bacteriol. 190: 7079-7086 [Abstract] [Full Text]  
  • Redko, Y., Courtin, P., Mezange, C., Huard, C., Chapot-Chartier, M.-P. (2007). Lactococcus lactis Gene yjgB Encodes a {gamma}-D-Glutaminyl-L-Lysyl- Endopeptidase Which Hydrolyzes Peptidoglycan. Appl. Environ. Microbiol. 73: 5825-5831 [Abstract] [Full Text]  
  • Carballido-Lopez, R. (2006). The Bacterial Actin-Like Cytoskeleton. Microbiol. Mol. Biol. Rev. 70: 888-909 [Abstract] [Full Text]  
  • Ahn, S.-J., Burne, R. A. (2006). The atlA Operon of Streptococcus mutans: Role in Autolysin Maturation and Cell Surface Biogenesis.. J. Bacteriol. 188: 6877-6888 [Abstract] [Full Text]  
  • Fukushima, T., Afkham, A., Kurosawa, S.-i., Tanabe, T., Yamamoto, H., Sekiguchi, J. (2006). A New D,L-Endopeptidase Gene Product, YojL (Renamed CwlS), Plays a Role in Cell Separation with LytE and LytF in Bacillus subtilis.. J. Bacteriol. 188: 5541-5550 [Abstract] [Full Text]  
  • Machata, S., Hain, T., Rohde, M., Chakraborty, T. (2005). Simultaneous Deficiency of both MurA and p60 Proteins Generates a Rough Phenotype in Listeria monocytogenes. J. Bacteriol. 187: 8385-8394 [Abstract] [Full Text]  
  • Serizawa, M., Sekiguchi, J. (2005). The Bacillus subtilis YdfHI two-component system regulates the transcription of ydfJ, a member of the RND superfamily. Microbiology 151: 1769-1778 [Abstract] [Full Text]  
  • Borges, F., Layec, S., Thibessard, A., Fernandez, A., Gintz, B., Hols, P., Decaris, B., Leblond-Bourget, N. (2005). cse, a Chimeric and Variable Gene, Encodes an Extracellular Protein Involved in Cellular Segregation in Streptococcus thermophilus. J. Bacteriol. 187: 2737-2746 [Abstract] [Full Text]  
  • Huard, C., Miranda, G., Redko, Y., Wessner, F., Foster, S. J., Chapot-Chartier, M.-P. (2004). Analysis of the Peptidoglycan Hydrolase Complement of Lactococcus lactis: Identification of a Third N-Acetylglucosaminidase, AcmC. Appl. Environ. Microbiol. 70: 3493-3499 [Abstract] [Full Text]  
  • Adu-Bobie, J., Lupetti, P., Brunelli, B., Granoff, D., Norais, N., Ferrari, G., Grandi, G., Rappuoli, R., Pizza, M. (2004). GNA33 of Neisseria meningitidis Is a Lipoprotein Required for Cell Separation, Membrane Architecture, and Virulence. Infect. Immun. 72: 1914-1919 [Abstract] [Full Text]  
  • Yamamoto, H., Kurosawa, S.-i., Sekiguchi, J. (2003). Localization of the Vegetative Cell Wall Hydrolases LytC, LytE, and LytF on the Bacillus subtilis Cell Surface and Stability of These Enzymes to Cell Wall-Bound or Extracellular Proteases. J. Bacteriol. 185: 6666-6677 [Abstract] [Full Text]  
  • Steen, A., Buist, G., Leenhouts, K. J., Khattabi, M. E., Grijpstra, F., Zomer, A. L., Venema, G., Kuipers, O. P., Kok, J. (2003). Cell Wall Attachment of a Widely Distributed Peptidoglycan Binding Domain Is Hindered by Cell Wall Constituents. J. Biol. Chem. 278: 23874-23881 [Abstract] [Full Text]  
  • Britton, R. A., Eichenberger, P., Gonzalez-Pastor, J. E., Fawcett, P., Monson, R., Losick, R., Grossman, A. D. (2002). Genome-Wide Analysis of the Stationary-Phase Sigma Factor (Sigma-H) Regulon of Bacillus subtilis. J. Bacteriol. 184: 4881-4890 [Abstract] [Full Text]  
  • Smith, T. J., Blackman, S. A., Foster, S. J. (2000). Autolysins of Bacillus subtilis: multiple enzymes with multiple functions. Microbiology 146: 249-262 [Full Text]  
  • Nugroho, F. A., Yamamoto, H., Kobayashi, Y., Sekiguchi, J. (1999). Characterization of a New Sigma-K-Dependent Peptidoglycan Hydrolase Gene That Plays a Role in Bacillus subtilis Mother Cell Lysis. J. Bacteriol. 181: 6230-6237 [Abstract] [Full Text]  
  • Kodama, T., Takamatsu, H., Asai, K., Kobayashi, K., Ogasawara, N., Watabe, K. (1999). The Bacillus subtilis yaaH Gene Is Transcribed by SigE RNA Polymerase during Sporulation, and Its Product Is Involved in Germination of Spores. J. Bacteriol. 181: 4584-4591 [Abstract] [Full Text]  
  • Kimura, Y., Takashima, Y., Tokumasu, Y., Sato, M. (1999). Molecular Cloning, Sequence Analysis, and Characterization of a Penicillin-Resistant DD-Carboxypeptidase of Myxococcus xanthus. J. Bacteriol. 181: 4696-4699 [Abstract] [Full Text]  
  • Ohnishi, R., Ishikawa, S., Sekiguchi, J. (1999). Peptidoglycan Hydrolase LytF Plays a Role in Cell Separation with CwlF during Vegetative Growth of Bacillus subtilis. J. Bacteriol. 181: 3178-3184 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»