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J Bacteriol, May 1998, p. 2574-2578, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification and Enzymatic Characterization of the Maltose-Inducible alpha -Glucosidase MalL (Sucrase-Isomaltase-Maltase) of Bacillus subtilis

Stefan Schönert, Thomas Buder, and Michael K. Dahl*

Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik, Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany

Received 29 December 1997/Accepted 3 March 1998

A gene coding for a putative alpha -glucosidase has been identified in the open reading frame yvdL (now termed malL), which was sequenced as part of the Bacillus subtilis genome project. The enzyme was overproduced in Escherichia coli and purified. Further analyses indicate that MalL is a specific oligo-1,4-1,6-alpha -glucosidase (sucrase-maltase-isomaltase). MalL expression in B. subtilis requires maltose induction and is subject to carbon catabolite repression by glucose and fructose. Insertional mutagenesis of malL resulted in a complete inactivation of the maltose-inducible alpha -glucosidase activity in crude protein extracts and a Mal- phenotype.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander Universität Erlangen-Nürnberg, Staudtstr. 5, D-91058 Erlangen, Germany. Phone: (49-9131) 858094. Fax: (49-9131) 858082. E-mail: mdahl{at}biologie.uni-erlangen.de.

J Bacteriol, May 1998, p. 2574-2578, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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