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Journal of Bacteriology, January 1999, p. 204-211, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evidence that the Extracytoplasmic Function Sigma Factor sigma E Is Required for Normal Cell Wall Structure in Streptomyces coelicolor A3(2)

Mark S. B. Paget,1,2,* Leony Chamberlin,1 Abdelmadjid Atrih,3 Simon J. Foster,3 and Mark J. Buttner1,2

John Innes Centre, Colney, Norwich NR4 7UH,1 School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ,2 and Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN,3 United Kingdom

Received 28 August 1998/Accepted 20 October 1998

The sigE gene of Streptomyces coelicolor A3(2) encodes an RNA polymerase sigma factor belonging to the extracytoplasmic function (ECF) subfamily. Constructed sigE deletion and disruption mutants were more sensitive than the parent to muramidases such as hen egg white lysozyme and to the CwlA amidase from Bacillus subtilis. This correlated with an altered muropeptide profile, as determined by reverse-phase high-performance liquid chromatography analysis of lytic digests of purified peptidoglycan. The sigE mutants required high levels of magnesium for normal growth and sporulation, overproducing the antibiotic actinorhodin and forming crenellated colonies in its absence. Together, these data suggest that sigE is required for normal cell wall structure. The role of sigma E was further investigated by analyzing the expression of hrdD, which is partially sigE dependent. The hrdD gene, which encodes the sigma HrdD subunit of RNA polymerase, is transcribed from two promoters, hrdDp1 and hrdDp2, both similar to promoters recognized by other ECF sigma factors. The activities of hrdDp1 and hrdDp2 were reduced 20- and 3-fold, respectively, in sigE mutants, although only hrdDp1 was recognized by Esigma E in vitro. Growth on media deficient in magnesium caused the induction of both hrdD promoters in a sigE-dependent manner.

* Corresponding author. Mailing address: John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom. Phone: (44) 1603 452571. Fax: (44) 1603 456844. E-mail: pagetm{at}bbsrc.ac.uk.

Journal of Bacteriology, January 1999, p. 204-211, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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