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Journal of Bacteriology, January 1999, p. 212-217, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification and Properties of the Genes Encoding Microcin E492 and Its Immunity Protein

Rosalba Lagos,* Jorge E. Villanueva,dagger and Octavio Monasterio

Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile

Received 18 May 1998/Accepted 29 October 1998

The gene coding for the immunity protein (mceB) and the structural gene of microcin E492 (mceA), a low-molecular-weight channel-forming bacteriocin produced by a strain of Klebsiella pneumoniae, have been characterized. The microcin gene codes for a precursor protein of either 99 or 103 amino acids. Protein sequencing of the N-terminal region of microcin E492 unequivocally identified this gene as the microcin structural gene and indicated that this microcin is synthesized as a precursor protein that is cleaved at either amino acid 15 or 19, at a site resembling the double-glycine motif. The gene encoding the 95-amino-acid immunity protein (mceB) was identified by cloning the DNA segment that encodes only this polypeptide into an expression vector and demonstrating the acquisition of immunity to microcin E492. As expected, the immunity protein was found to be associated with the inner membrane. Analysis of the DNA sequence indicates that these genes belong to the same family as microcin 24, and they do not share structural motifs with any other known channel-forming bacteriocin. The organization of the microcin- and immunity protein-encoding genes suggests that they are coordinately expressed.

* Corresponding author. Mailing address: Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile. Phone: 56-2-678-7338. Fax: 56-2-271-3891. E-mail: rolagos{at}abello.dic.uchile.cl.

dagger Permanent address: Facultad de Ciencias, Campus San Andrés, Universidad Católica de la Santísima Concepción, Concepción, Chile.

Journal of Bacteriology, January 1999, p. 212-217, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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