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Journal of Bacteriology, May 1999, p. 3051-3057, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

MHY1 Encodes a C2H2-Type Zinc Finger Protein That Promotes Dimorphic Transition in the Yeast Yarrowia lipolytica

Cleofe A. R. Hurtado and Richard A. Rachubinski*

Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada

Received 29 January 1999/Accepted 11 March 1999

The yeast-to-hypha morphological transition (dimorphism) is typical of many pathogenic fungi. Dimorphism has been attributed to changes in temperature and nutritional status and is believed to constitute a mechanism of response to adverse conditions. We have isolated and characterized a gene, MHY1, whose transcription is dramatically increased during the yeast-to-hypha transition in Yarrowia lipolytica. Deletion of MHY1 is viable and has no effect on mating, but it does result in a complete inability of cells to undergo mycelial growth. MHY1 encodes a C2H2-type zinc finger protein, Mhy1p, which can bind putative cis-acting DNA stress response elements, suggesting that Mhy1p may act as a transcription factor. Interestingly, Mhy1p tagged with a hemagglutinin epitope was concentrated in the nuclei of actively growing cells found at the hyphal tip.

* Corresponding author. Mailing address: Department of Cell Biology, University of Alberta, Medical Sciences Building 5-14, Edmonton, Alberta T6G 2H7, Canada. Phone: (780) 492-9868. Fax: (780) 492-9278. E-mail: Rick.Rachubinski{at}ualberta.ca.

Journal of Bacteriology, May 1999, p. 3051-3057, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jaafar, L., Zueco, J. (2004). Characterization of a glycosylphosphatidylinositol-bound cell-wall protein (GPI-CWP) in Yarrowia lipolytica. Microbiology 150: 53-60 [Abstract] [Full Text]  
  • Hurtado, C. A. R., Rachubinski, R. A. (2002). YlBMH1 encodes a 14-3-3 protein that promotes filamentous growth in the dimorphic yeast Yarrowia lipolytica. Microbiology 148: 3725-3735 [Abstract] [Full Text]  
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  • Richard, M., Quijano, R. R., Bezzate, S., Bordon-Pallier, F., Gaillardin, C. (2001). Tagging Morphogenetic Genes by Insertional Mutagenesis in the Yeast Yarrowia lipolytica. J. Bacteriol. 183: 3098-3107 [Abstract] [Full Text]  
  • Hurtado, C. A. R., Beckerich, J.-M., Gaillardin, C., Rachubinski, R. A. (2000). A Rac Homolog Is Required for Induction of Hyphal Growth in the Dimorphic Yeast Yarrowia lipolytica. J. Bacteriol. 182: 2376-2386 [Abstract] [Full Text]  


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