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Journal of Bacteriology, May 1999, p. 3277-3280, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Histidinol Phosphate Phosphatase, Catalyzing the Penultimate Step of the Histidine Biosynthesis Pathway, Is Encoded by ytvP (hisJ) in Bacillus subtilis

Dominique le Coq,* Sabine Fillinger, and Stéphane Aymerich

Laboratoire de Génétique Moléculaire et Cellulaire, Centre National de la Recherche Scientifique ERS 567, Institut National de la Recherche Agronomique, F-78850 Thiverval-Grignon, France

Received 5 November 1998/Accepted 23 February 1999

The deduced product of the Bacillus subtilis ytvP gene is similar to that of ORF13, a gene of unknown function in the Lactococcus lactis histidine biosynthesis operon. A B. subtilis ytvP mutant was auxotrophic for histidine. The only enzyme of the histidine biosynthesis pathway that remained uncharacterized in B. subtilis was histidinol phosphate phosphatase (HolPase), catalyzing the penultimate step of this pathway. HolPase activity could not be detected in crude extracts of the ytvP mutant, while purified glutathione S-transferase-YtvP fusion protein exhibited strong HolPase activity. These observations demonstrated that HolPase is encoded by ytvP in B. subtilis and led us to rename this gene hisJ. Together with the HolPase of Saccharomyces cerevisiae and the presumed HolPases of L. lactis and Schizosaccharomyces pombe, HisJ constitutes a family of related enzymes that are not homologous to the HolPases of Escherichia coli, Salmonella typhimurium, and Haemophilus influenzae.

* Corresponding author. Mailing address: Laboratoire de GénétiqueMoléculaire et Cellulaire, Centre National de la Recherche Scientifique ERS 567, Institut National de la Recherche Agronomique, F-78850 Thiverval-Grignon, France. Phone: (33) 1.30.81.54.45. Fax: (33) 1.30.81.54.57. E-mail: lecoq{at}platon.grignon.inra.fr.

Journal of Bacteriology, May 1999, p. 3277-3280, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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