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Journal of Bacteriology, May 1999, p. 3281-3283, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Bacteriocin Release Protein Triggers Dimerization of Outer Membrane Phospholipase A In Vivo

Niek Dekker,1,* Jan Tommassen,2 and Hubertus M. Verheij1,dagger

Department of Enzymology and Protein Engineering, Center for Biomembranes and Lipid Enzymology,1 and Department of Molecular Cell Biology,2 Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands

Received 13 January 1999/Accepted 15 March 1999

Bacteriocin release protein is known to activate outer membrane phospholipase A (OMPLA), which results in the release of colicin from Escherichia coli. In vivo chemical cross-linking experiments revealed that the activation coincides with dimerization of OMPLA. Permeabilization of the cell envelope and dimerization were characterized by a lag time of 2 h.

* Corresponding author. Mailing address: Department of Enzymology and Protein Engineering, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. Phone: 31-30-2532458. Fax: 31-30-2522478. E-mail: n.dekker{at}chem.uu.nl.

dagger Hubertus M. Verheij passed away in a tragic accident on 1 August 1998.

Journal of Bacteriology, May 1999, p. 3281-3283, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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