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Journal of Bacteriology, June 1999, p. 3810-3815, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Role for a Highly Conserved Protein of Unknown Function in Regulation of Bacillus subtilis purA by the Purine Repressor

Pekka Rappu,1,* Byung Sik Shin,2,dagger Howard Zalkin,2 and Pekka Mäntsälä1

Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland,1 and Department of Biochemistry, Purdue University, West Lafayette, Indiana 479072

Received 25 March 1998/Accepted 14 April 1999

Regulation of the purine biosynthetic gene purA was examined by using a transcriptional fusion to a luciferase reporter gene. Transcription was repressed about 10-fold by the addition of adenine and increased approximately 4.5-fold by the addition of guanosine. This regulation is mediated by a purine repressor (PurR). In a purR mutant, basal expression was increased 10-fold, and there was no further stimulation by guanosine or repression by adenine. An open reading frame, yabJ, immediately downstream from purR was found to have a role in the repression of purA by adenine. Repression by adenine was perturbed in a purR+ yabJ mutant, although guanosine regulation was retained. Mutations in the PurR PRPP binding motif abolished guanosine regulation in the yabJ mutant. Thus, PRPP appears to be required for upregulation by guanosine. The amino acid sequence of YabJ is homologous to the YER057c/YjgF protein family of unknown function.

* Corresponding author. Mailing address: Department of Biochemistry and Food Chemistry, University of Turku, Vatselankatu 2, FIN-20014 Turku, Finland. Phone: 358-2 333 6856. Fax: 358-2 333 6860. E-mail: pekrappu{at}utu.fi.

dagger Present address: Bacterial Molecular Genetics Research Unit, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon 305-600, Korea.

Journal of Bacteriology, June 1999, p. 3810-3815, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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