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Journal of Bacteriology, June 1999, p. 3824-3829, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

relA Is Required for Actinomycin Production in Streptomyces antibioticus

Shannan Hoyt and George H. Jones*

Department of Biology, Emory University, Atlanta, Georgia 30322

Received 23 July 1998/Accepted 16 April 1999

The relA gene from Streptomyces antibioticus has been cloned and sequenced. The gene encodes a protein with an Mr of 93,653, which is 91% identical to the corresponding protein from Streptomyces coelicolor. Disruption of S. antibioticus relA produces a strain which grows significantly more slowly on actinomycin production medium than the wild type or a disruptant to which the intact relA gene was restored. Moreover, the disruptant was unable to accumulate ppGpp to the levels observed during the normal course of growth and actinomycin production in the wild type. The strain containing the disrupted relA gene did not produce actinomycin and contained significantly lower levels of the enzyme phenoxazinone synthase than the wild-type strain. Actinomycin synthetase I, a key enzyme in the actinomycin biosynthetic pathway, was undetectable in the relA disruptant. Growth of the disruptant on low-phosphate medium did not restore actinomycin production.

* Corresponding author. Mailing address: Department of Biology, 1510 Clifton Rd., Emory University, Atlanta, GA 30322. Phone: (404) 727-0712. Fax: (404) 727-2880. E-mail: gjones{at}biology.emory.edu.

Journal of Bacteriology, June 1999, p. 3824-3829, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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