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Journal of Bacteriology, June 1999, p. 3830-3832, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

AGT1, Encoding an alpha -Glucoside Transporter Involved in Uptake and Intracellular Accumulation of Trehalose in Saccharomyces cerevisiae

Lucile Plourde-Owobi,1 Sophie Durner,1 Jean-Luc Parrou,1 Roman Wieczorke,2 Gerard Goma,1 and Jean François1,*

Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, LA INRA, Institut National des Sciences Appliquées, 31077 Toulouse, France,1 and Institut für Mikrobiologie, Heinrich-Heine-Universität, D-40225 Düsseldorf, Germany2

Received 23 November 1998/Accepted 13 April 1999

The trehalose content in Saccharomyces cerevisiae can be significantly manipulated by including trehalose at an appropriate level in the growth medium. Its uptake is largely dependent on the expression of AGT1, which encodes an alpha -glucoside transporter. The trehalose found in a tps1 mutant of trehalose synthase may therefore largely reflect its uptake from the enriched medium that was employed.


* Corresponding author. Mailing address: Département Génie Biochimique et Alimentaire, Institut National des Sciences Appliquées, Complexe Scientifique de Rangeuil, 31077 Toulouse Cedex 04, France. Phone: 33 5 61 55 94 92. Fax: 33 5 61 55 94 00. E-mail: fran_jm{at}insa-tlse.fr.


Journal of Bacteriology, June 1999, p. 3830-3832, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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