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Journal of Bacteriology, June 1999, p. 3860-3863, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genotype, Phenotype, and Protein Structure in a Regulator of Sporulation: Effects of Mutations in the spoIIAA Gene of Bacillus subtilis

Daniela Barillà,dagger Isabelle Lucet, Anne Kuhlmann,Dagger and Michael D. Yudkin*

Microbiology Unit, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom

Received 10 November 1998/Accepted 11 April 1999

SpoIIAA, a phosphorylatable protein, is essential to the regulation of sigma F, the first sporulation-specific transcription factor of Bacillus subtilis. The solution structure of SpoIIAA has recently been published. Here we examine four mutant SpoIIAA proteins and correlate their properties with the phenotypes of the corresponding B. subtilis mutant strains. Two of the mutations severely disrupted the structure of the protein, a third greatly diminished the rate of its phosphorylation and abolished dephosphorylation, and the fourth left phosphorylation unaffected but reduced the rate of dephosphorylation about 10-fold.

* Corresponding author. Mailing address: Microbiology Unit, Department of Biochemistry, University of Oxford, South Parks Rd., Oxford OX1 3QU, United Kingdom. Phone: 44 1865 275302. Fax: 44 1865 275297. E-mail: mdy{at}bioch.ox.ac.uk.

dagger Present address: Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.

Dagger Present address: Philipps-Universität Marburg, Fachbereich Biologie, Laboratorium für Mikrobiologie, D-35032 Marburg, Germany.

Journal of Bacteriology, June 1999, p. 3860-3863, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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