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Journal of Bacteriology, July 1999, p. 4098-4102, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Cloning and Functional Expression of Alternative Oxidase from Candida albicans

Won-Ki Huh and Sa-Ouk Kang*

Laboratory of Biophysics, Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Seoul 151-742, Republic of Korea

Received 29 December 1998/Accepted 18 April 1999

The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions but could still induce cyanide-resistant respiration when treated with antimycin A. The measurement of respiratory activity and Western blot analysis suggested the presence of another AOX. When C. albicans AOX1 was expressed in alternative oxidase-deficient Saccharomyces cerevisiae, it could confer cyanide-resistant respiration on S. cerevisiae.

* Corresponding author. Mailing address: Laboratory of Biophysics, Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Seoul 151-742, Republic of Korea. Phone: (82) (2) 880 6703. Fax: (82) (2) 888 4911. E-mail: kangsaou{at}plaza.snu.ac.kr.

Journal of Bacteriology, July 1999, p. 4098-4102, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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