This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kakinuma, Y.
Right arrow Articles by Igarashi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kakinuma, Y.
Right arrow Articles by Igarashi, K.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 1999, p. 4103-4105, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation and Properties of Enterococcus hirae Mutants Defective in the Potassium/Proton Antiport System

Yoshimi Kakinuma* and Kazuei Igarashi

Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263-8522, Japan

Received 6 January 1999/Accepted 28 April 1999

A K+/H+ antiporter regulates cytoplasmic pH in Enterococcus hirae growing at alkaline pH. Mutants defective in this antiport activity were alkaline pH sensitive. One mutant, Pop1, lacked both K+/methylamine exchange at pH 9.5 and concomitant acidification of cytoplasmic pH. Pop1 grew well at pHs below 8 but did not at pHs above 9, conditions under which cytoplasmic pH was not fully acidified.


* Corresponding author. Mailing address: Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan. Phone: 81-43-290-2898. Fax: 81-43-290-2900. E-mail: yoshimi{at}athenaeum.p.chiba-u.ac.jp.


Journal of Bacteriology, July 1999, p. 4103-4105, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Sawatari, Y., Yokota, A. (2007). Diversity and Mechanisms of Alkali Tolerance in Lactobacilli. Appl. Environ. Microbiol. 73: 3909-3915 [Abstract] [Full Text]  
  • Radchenko, M. V., Tanaka, K., Waditee, R., Oshimi, S., Matsuzaki, Y., Fukuhara, M., Kobayashi, H., Takabe, T., Nakamura, T. (2006). Potassium/Proton Antiport System of Escherichia coli. J. Biol. Chem. 281: 19822-19829 [Abstract] [Full Text]