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Journal of Bacteriology, July 1999, p. 4110-4113, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification and Expression of the Genes Encoding a Reactivating Factor for Adenosylcobalamin-Dependent Glycerol Dehydratase

Takamasa Tobimatsu, Hideki Kajiura, Michio Yunoki, Muneaki Azuma, and Tetsuo Toraya*

Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan

Received 18 February 1999/Accepted 27 April 1999

Adenosylcobalamin-dependent glycerol dehydratase undergoes inactivation by glycerol, the physiological substrate, during catalysis. In permeabilized cells of Klebsiella pneumoniae, the inactivated enzyme is reactivated in the presence of ATP, Mg2+, and adenosylcobalamin. We identified the two open reading frames as the genes for a reactivating factor for glycerol dehydratase and designated them gdrA and gdrB. The reactivation of the inactivated glycerol dehydratase by the gene products was confirmed in permeabilized recombinant Escherichia coli cells coexpressing GdrA and GdrB proteins with glycerol dehydratase.

* Corresponding author. Mailing address: Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan. Phone: 81-86-251-8194. Fax: 81-86-251-8264. E-mail: toraya{at}biotech.okayama-u.ac.jp.

Journal of Bacteriology, July 1999, p. 4110-4113, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Kajiura, H., Mori, K., Tobimatsu, T., Toraya, T. (2001). Characterization and Mechanism of Action of a Reactivating Factor for Adenosylcobalamin-dependent Glycerol Dehydratase. J. Biol. Chem. 276: 36514-36519 [Abstract] [Full Text]  


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