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 Previous Article

Journal of Bacteriology, July 1999, p. 4137-4141, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Cloning and Characterization of a Locus Responsible for O Acetylation of the O Polysaccharide of Legionella pneumophila Serogroup 1 Lipopolysaccharide

Chang Hua Zou,1 Yuriy A. Knirel,2,3 Jürgen H. Helbig,4 Ulrich Zähringer,2 and Clifford S. Mintz1,*

Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida, 331011; N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 117913 Moscow, Russian Federation3; and Forschungszentrum Borstel, Zentrum für Experimentelle Biologie und Biowissenschaften, D-23845 Borstel,2 and Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technische Universität Dresden, D-01307 Dresden,4 Germany

Received 1 February 1999/Accepted 26 April 1999

Complementation experiments, Tn5 mutagenesis, and DNA sequencing were used to identify a locus (lag-1) that participates in acetylation of Legionella pneumophila serogroup 1 lipopolysaccharide. Nuclear magnetic resonance analyses of lipopolysaccharides from mutant and complemented strains suggest that lag-1 is responsible for O acetylation of serogroup 1 O polysaccharide.

* Corresponding author. Present address: BioInsights, Inc., 153 Dorchester Dr., East Windsor, NJ 08520. Phone: (609) 426-1751. Fax: (609) 426-4530. E-mail: cmintz{at}home.com.

Journal of Bacteriology, July 1999, p. 4137-4141, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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