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Journal of Bacteriology, July 1999, p. 4161-4169, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

First Chromosomal Restriction Map of Actinobacillus pleuropneumoniae and Localization of Putative Virulence-Associated Genes

Winfried Oswald,* Denis V. Konine, Judith Rohde, and Gerald-F. Gerlach

Tierärztliche Hochschule Hannover, Institut für Mikrobiologie und Tierseuchen, 30173 Hannover, Germany

Received 12 January 1999/Accepted 5 May 1999

Combined physical and genetic maps of the genomes of Actinobacillus pleuropneumoniae AP76 (serotype 7 clinical isolate) and of A. pleuropneumoniae ATCC 27088 (serotype 1 reference strain) were constructed by using the restriction endonucleases ApaI, AscI, NotI, and SalI. The chromosome sizes as determined by the addition of estimated fragment sizes were 2.4 Mbp, and both maps had a resolution of approximately 100 kbp. The linkages between the ApaI, AscI, NotI, and SalI fragments and their relative positions were determined by (i) fragment excision and redigestion and (ii) partial digests of defined fragments and Southern blot using end-standing probes. The single SalI site within the chromosome of strain A. pleuropneumoniae AP76 was defined as position 1 of the map; for the map of A. pleuropneumoniae ATCC 27088, the corresponding SalI site was chosen. Putative virulence-associated genes (apx, omlA, sodA, tbpBA, ureC, and a repeat element) and housekeeping genes (glyA, metJ, recA, and rhoAP) were positioned on the physical maps and located on the ApaI and NotI fragments of A. pleuropneumoniae serotype reference strains.

* Corresponding author. Mailing address: Tierärztliche Hochschule Hannover, Institut für Mikrobiologie und Tierseuchen, Bischofsholer Damm 15, 30173 Hannover, Germany. Phone: 49-511-856-7206. Fax: 49-511-856-7697. E-mail: woswald{at}micro.tiho-hannover.de.

Journal of Bacteriology, July 1999, p. 4161-4169, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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