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Journal of Bacteriology, July 1999, p. 4245-4249, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Site-Specific Recombination of Bacteriophage P22 Does Not Require Integration Host Factor

Eun Hee Cho,1,* Chan-Eun Nam,2 Renato Alcaraz Jr.,3 and Jeffrey F. Gardner3

Department of Science Education1 and Department of Genetic Engineering,2 Chosun University, Kwangju 501-759, Korea, and Department of Microbiology, University of Illinois, Urbana, Illinois 618013

Received 2 August 1998/Accepted 31 March 1999

Site-specific recombination by phages lambda  and P22 is carried out by multiprotein-DNA complexes. Integration host factor (IHF) facilitates lambda  site-specific recombination by inducing DNA bends necessary to form an active recombinogenic complex. Mutants lacking IHF are over 1,000-fold less proficient in supporting lambda  site-specific recombination than wild-type cells. Although the attP region of P22 contains strong IHF binding sites, in vivo measurements of integration and excision frequencies showed that infecting P22 phages can perform site-specific recombination to its maximum efficiency in the absence of IHF. In addition, a plasmid integration assay showed that integrative recombination occurs equally well in wild-type and ihfA mutant cells. P22 integrative recombination is also efficient in Escherichia coli in the absence of functional IHF. These results suggest that nucleoprotein structures proficient for recombination can form in the absence of IHF or that another factor(s) can substitute for IHF in the formation of complexes.

* Corresponding author. Mailing address: Department of Science Education, Chosun University, Dong-gu, Seosuk-dong 375, Kwangju 501-759, Korea. Phone: 82-62-230-7370. Fax: 82-62-232-8122. E-mail: ehcho{at}chosun.ac.kr.

Journal of Bacteriology, July 1999, p. 4245-4249, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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