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Journal of Bacteriology, July 1999, p. 4353-4364, Vol. 181, No. 14
Centro Nacional de Biotecnología,
Consejo Superior de Investigaciones Científicas, Campus
Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain
Received 3 March 1999/Accepted 5 May 1999
The sequence of a 4.8-kbp DNA fragment adjacent to the right-hand
end of the actinorhodin biosynthetic (act) cluster
downstream of actVB-orf6 from Streptomyces
coelicolor A3(2) reveals six complete open reading frames, named
orf7 to orf12. The deduced amino acid sequences
from orf7, orf10, and orf11 show
significant similarities with the following products in the databases:
a putative protein from the S. coelicolor SCP3 plasmid,
LysR-type transcriptional regulators, and proteins belonging to the
family of short-chain dehydrogenases/reductases, respectively. The
deduced product of orf8 reveals low similarities with
several methyltransferases from different sources, while
orf9 and orf12 products show no similarities
with other known proteins. Disruptions of orf10 and orf11 genes in S. coelicolor appear to have no
significant effect on the production of actinorhodin. Nevertheless,
disruption or deletion of orf10 in Streptomyces
lividans causes actinorhodin overproduction. The introduction of
extra copies of orf10 and orf11 genes in an
S. coelicolor actIII mutant restores the ability to produce
actinorhodin. Transcriptional analysis and DNA footprinting indicate that Orf10 represses its own transcription and regulates orf11 transcription, expression of which might require the
presence of an unknown inducer. No DNA target for Orf10 protein was
found within the act cluster.
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
An Additional Regulatory Gene for Actinorhodin
Production in Streptomyces lividans Involves a LysR-Type
Transcriptional Regulator
*
Corresponding author. Mailing address: Centro Nacional
de Biotecnología, CSIC, Campus Universidad Autónoma de
Madrid, Cantoblanco, 28049 Madrid, Spain. Phone: 34-91-5854548. Fax:
34-91-5854506. E-mail: fmalpart{at}cnb.uam.es.
Journal of Bacteriology, July 1999, p. 4353-4364, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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