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Journal of Bacteriology, July 1999, p. 4437-4440, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Water Transport across Yeast Vacuolar and Plasma Membrane-Targeted Secretory Vesicles Occurs by Passive Diffusion

Larry A. Coury,1 Mark Hiller,2 John C. Mathai,1 Elizabeth W. Jones,2 Mark L. Zeidel,1 and Jeffrey L. Brodsky3,*

Laboratory of Epithelial Cell Biology, Renal Electrolyte Division, Department of Medicine, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213-25001; Department of Biological Sciences, Mellon Institute, Carnegie Mellon University, Pittsburgh, Pennsylvania 152132; and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 152603

Received 7 December 1998/Accepted 10 May 1999

To determine whether solute transport across yeast membranes was facilitated, we measured the water and solute permeations of vacuole-derived and late secretory vesicles in Saccharomyces cerevisiae; all permeations were consistent with passive diffusive flow. We also overexpressed Fps1p, the putative glycerol facilitator in S. cerevisiae, in secretory vesicles but observed no effect on water, glycerol, formamide, or urea permeations. However, spheroplasts prepared from the strain overexpressing Fps1p showed enhanced glycerol uptake, suggesting that Fps1p becomes active only upon insertion in the plasma membrane.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260. Phone: (412) 624-4831. Fax: (412) 624-4759. E-mail: jbrodsky+{at}pitt.edu.

Journal of Bacteriology, July 1999, p. 4437-4440, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Hohmann, S. (2002). Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol. Mol. Biol. Rev. 66: 300-372 [Abstract] [Full Text]  


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