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Journal of Bacteriology, August 1999, p. 4549-4553, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Illegitimate Recombination Induced by Overproduction of DnaB Helicase in Escherichia coli

Teruhito Yamashita, Katsuhiro Hanada, Mihoko Iwasaki, Hirotaka Yamaguchi, and Hideo Ikeda*

Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan

Received 29 October 1998/Accepted 22 May 1999

Illegitimate recombination that usually takes place at a low frequency is greatly enhanced by treatment with DNA-damaging agents. It is thought that DNA double-strand breaks induced by this DNA damage are important for initiation of illegitimate recombination. Here we show that illegitimate recombination is enhanced by overexpression of the DnaB protein in Escherichia coli. The recombination enhanced by DnaB overexpression occurred between short regions of homology. We propose a model for the initiation of illegitimate recombination in which DnaB overexpression may excessively unwind DNA at replication forks and induce double-strand breaks, resulting in illegitimate recombination. The defect in RecQ has a synergistic effect on the increased illegitimate recombination in cells containing the overproduced DnaB protein, implying that DnaB works in the same pathway as RecQ does but that they work at different steps.

* Corresponding author. Present address: Center for Basic Research, The Kitasato Institute, Shirokane 5-9-1, Minato-ku, Tokyo 108-8642, Japan. Phone: 81-3-5791-6324. Fax: 81-3-5689-6270. E-mail: ike{at}u-tokyo.ac.jp.

Journal of Bacteriology, August 1999, p. 4549-4553, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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