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Journal of Bacteriology, August 1999, p. 4661-4664, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Characterization of Genetic Loci Required for Secretion of Exoproducts in Burkholderia pseudomallei

David DeShazer,1 Paul J. Brett,2 Mary N. Burtnick,2 and Donald E. Woods2,*

Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1,2 and Genetics and Physiology Branch, Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 217021

Received 15 March 1999/Accepted 5 May 1999

Previous studies have demonstrated that Burkholderia pseudomallei secretes protease, lipase, and phospholipase C (PLC) into the extracellular milieu, but their mechanisms of secretion and roles in pathogenesis have not been elucidated. In this study, we isolated and characterized 29 transposon mutants unable to secrete protease, lipase, and PLC.

* Corresponding author. Mailing address: Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta T2N 4N1, Canada. Phone: (403) 220-2564. Fax: (403) 283-5241. E-mail: woods{at}acs.ucalgary.ca.

Journal of Bacteriology, August 1999, p. 4661-4664, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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