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Journal of Bacteriology, August 1999, p. 4680-4685, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Complementation of Conjugation Functions of Streptomyces lividans Plasmid pIJ101 by the Related Streptomyces Plasmid pSB24.2

Gregg S. Pettis* and Shubha Prakash

Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803

Received 29 March 1999/Accepted 28 May 1999

A database search revealed extensive sequence similarity between Streptomyces lividans plasmid pIJ101 and Streptomyces plasmid pSB24.2, which is a deletion derivative of Streptomyces cyanogenus plasmid pSB24.1. The high degree of relatedness between the two plasmids allowed the construction of a genetic map of pSB24.2, consisting of putative transfer and replication loci. Two pSB24.2 loci, namely, the cis-acting locus for transfer (clt) and the transfer-associated korB gene, were shown to be capable of complementing the pIJ101 clt and korB functions, respectively, a result that is consistent with the notion that pIJ101 and the parental plasmid pSB24.1 encode highly similar, if not identical, conjugation systems.

* Corresponding author. Mailing address: Department of Biological Sciences, Louisiana State University, 508 Life Sciences Building, Baton Rouge, LA 70803. Phone: (225) 388-2798. Fax: (225) 388-2597. E-mail: gpettis{at}unix1.sncc.lsu.edu.

Journal of Bacteriology, August 1999, p. 4680-4685, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ducote, M. J., Pettis, G. S. (2003). Common and Distinguishing Regulatory and Expression Characteristics of the Highly Related KorB Proteins of Streptomycete Plasmids pIJ101 and pSB24.2. J. Bacteriol. 185: 3780-3787 [Abstract] [Full Text]  
  • Zhang, X., Clark, C. A., Pettis, G. S. (2003). Interstrain Inhibition in the Sweet Potato Pathogen Streptomyces ipomoeae: Purification and Characterization of a Highly Specific Bacteriocin and Cloning of Its Structural Gene. Appl. Environ. Microbiol. 69: 2201-2208 [Abstract] [Full Text]  
  • Pettis, G. S., Ward, N., Schully, K. L. (2001). Expression Characteristics of the Transfer-Related kilB Gene Product of Streptomyces Plasmid pIJ101: Implications for the Plasmid Spread Function. J. Bacteriol. 183: 1339-1345 [Abstract] [Full Text]  
  • Ducote, M. J., Prakash, S., Pettis, G. S. (2000). Minimal and Contributing Sequence Determinants of the cis-Acting Locus of Transfer (clt) of Streptomycete Plasmid pIJ101 Occur within an Intrinsically Curved Plasmid Region. J. Bacteriol. 182: 6834-6841 [Abstract] [Full Text]  


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