This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yorimitsu, T.
Right arrow Articles by Homma, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yorimitsu, T.
Right arrow Articles by Homma, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 1999, p. 5103-5106, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Functional Interaction between PomA and PomB, the Na+-Driven Flagellar Motor Components of Vibrio alginolyticus

Tomohiro Yorimitsu, Ken Sato, Yukako Asai, Ikuro Kawagishi, and Michio Homma*

Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-Ku, Nagoya 464-8602, Japan

Received 1 March 1999/Accepted 21 May 1999

Four proteins, PomA, PomB, MotX, and MotY, appear to be involved in force generation of the sodium-driven polar flagella of Vibrio alginolyticus. Among these, PomA and PomB seem to be associated and to form a sodium channel. By using antipeptide antibodies against PomA or PomB, we carried out immunoprecipitation to verify whether these proteins form a complex and examined the in vivo stabilities of PomA and PomB. As a result, we could demonstrate that PomA and PomB functionally interact with each other.

* Corresponding author. Mailing address: Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-Ku, Nagoya 464-8602, Japan. Phone: 81-52-789-2991. Fax: 81-52-789-3001. E-mail: g44416a{at}nucc.cc.nagoya-u.ac.jp.

Journal of Bacteriology, August 1999, p. 5103-5106, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Van Dellen, K. L., Houot, L., Watnick, P. I. (2008). Genetic Analysis of Vibrio cholerae Monolayer Formation Reveals a Key Role for {Delta}{Psi} in the Transition to Permanent Attachment. J. Bacteriol. 190: 8185-8196 [Abstract] [Full Text]  
  • Terashima, H., Abe-Yoshizumi, R., Kojima, S., Homma, M. (2008). Cell-free Synthesis of the Torque-Generating Membrane Proteins, PomA and PomB, of the Na+-driven Flagellar Motor in Vibrio alginolyticus. J Biochem 144: 635-642 [Abstract] [Full Text]  
  • Obara, M., Yakushi, T., Kojima, S., Homma, M. (2008). Roles of Charged Residues in the C-Terminal Region of PomA, a Stator Component of the Na+-Driven Flagellar Motor. J. Bacteriol. 190: 3565-3571 [Abstract] [Full Text]  
  • Yagasaki, J., Okabe, M., Kurebayashi, R., Yakushi, T., Homma, M. (2006). Roles of the Intramolecular Disulfide Bridge in MotX and MotY, the Specific Proteins for Sodium-Driven Motors in Vibrio spp.. J. Bacteriol. 188: 5308-5314 [Abstract] [Full Text]  
  • Hizukuri, Y., Yakushi, T., Kawagishi, I., Homma, M. (2006). Role of the Intramolecular Disulfide Bond in FlgI, the Flagellar P-Ring Component of Escherichia coli.. J. Bacteriol. 188: 4190-4197 [Abstract] [Full Text]  
  • Kusumoto, A., Kamisaka, K., Yakushi, T., Terashima, H., Shinohara, A., Homma, M. (2006). Regulation of Polar Flagellar Number by the flhF and flhG Genes in Vibrio alginolyticus. J Biochem 139: 113-121 [Abstract] [Full Text]  
  • Okabe, M., Yakushi, T., Homma, M. (2005). Interactions of MotX with MotY and with the PomA/PomB Sodium Ion Channel Complex of the Vibrio alginolyticus Polar Flagellum. J. Biol. Chem. 280: 25659-25664 [Abstract] [Full Text]  
  • Yakushi, T., Hattori, N., Homma, M. (2005). Deletion Analysis of the Carboxyl-Terminal Region of the PomB Component of the Vibrio alginolyticus Polar Flagellar Motor. J. Bacteriol. 187: 778-784 [Abstract] [Full Text]  
  • Fukuoka, H., Yakushi, T., Homma, M. (2004). Concerted Effects of Amino Acid Substitutions in Conserved Charged Residues and Other Residues in the Cytoplasmic Domain of PomA, a Stator Component of Na+-Driven Flagella. J. Bacteriol. 186: 6749-6758 [Abstract] [Full Text]  
  • Yakushi, T., Maki, S., Homma, M. (2004). Interaction of PomB with the Third Transmembrane Segment of PomA in the Na+-Driven Polar Flagellum of Vibrio alginolyticus. J. Bacteriol. 186: 5281-5291 [Abstract] [Full Text]  
  • Yakushi, T., Kojima, M., Homma, M. (2004). Isolation of Vibrio alginolyticus sodium-driven flagellar motor complex composed of PomA and PomB solubilized by sucrose monocaprate. Microbiology 150: 911-920 [Abstract] [Full Text]  
  • Yorimitsu, T., Kojima, M., Yakushi, T., Homma, M. (2004). Multimeric Structure of the PomA/PomB Channel Complex in the Na+-Driven Flagellar Motor of Vibrio alginolyticus. J Biochem 135: 43-51 [Abstract] [Full Text]  
  • McCarter, L. L. (2001). Polar Flagellar Motility of the Vibrionaceae. Microbiol. Mol. Biol. Rev. 65: 445-462 [Abstract] [Full Text]  
  • Kojima, S., Shoji, T., Asai, Y., Kawagishi, I., Homma, M. (2000). A Slow-Motility Phenotype Caused by Substitutions at Residue Asp31 in the PomA Channel Component of a Sodium-Driven Flagellar Motor. J. Bacteriol. 182: 3314-3318 [Abstract] [Full Text]  
  • Sato, K., Homma, M. (2000). Functional Reconstitution of the Na+-driven Polar Flagellar Motor Component of Vibrio alginolyticus. J. Biol. Chem. 275: 5718-5722 [Abstract] [Full Text]  
  • Asai, Y., Shoji, T., Kawagishi, I., Homma, M. (2000). Cysteine-Scanning Mutagenesis of the Periplasmic Loop Regions of PomA, a Putative Channel Component of the Sodium-Driven Flagellar Motor in Vibrio alginolyticus. J. Bacteriol. 182: 1001-1007 [Abstract] [Full Text]  
  • Asai, Y., Kawagishi, I., Sockett, R. E., Homma, M. (1999). Hybrid Motor with H+- and Na+-Driven Components Can Rotate Vibrio Polar Flagella by Using Sodium Ions. J. Bacteriol. 181: 6332-6338 [Abstract] [Full Text]  
  • Yorimitsu, T., Asai, Y., Sato, K., Homma, M. (2000). Intermolecular Cross-linking between the Periplasmic Loop3-4 Regions of PomA, a Component of the Na+-driven Flagellar Motor of Vibrio alginolyticus. J. Biol. Chem. 275: 31387-31391 [Abstract] [Full Text]  
  • Sato, K., Homma, M. (2000). Multimeric Structure of PomA, a Component of the Na+-driven Polar Flagellar Motor of Vibrio alginolyticus. J. Biol. Chem. 275: 20223-20228 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»