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Journal of Bacteriology, August 1999, p. 5114-5118, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

In Vivo Observation of Cell Division of Anaerobic Hyperthermophiles by Using a High-Intensity Dark-Field Microscope

Christian Horn,1 Bernd Paulmann,1 Gertraude Kerlen,2 Norbert Junker,3 and Harald Huber1,*

Archaeenzentrum, Universität Regensburg, 93053 Regensburg,1 Institut für den wissenschaftlichen Film, 37075 Göttingen,2 and Olympus Optical Co. (Europe) GmbH, 20097 Hamburg,3 Germany

Received 22 January 1999/Accepted 3 June 1999

To study growth and cell division of anaerobic hyperthermophilic archaea in vivo, a cultivation technique using glass capillaries was developed. At temperatures of 90 to 98°C, at least 10 successive cell divisions of Pyrodictium abyssi TAG 11 were documented. Cells divide by binary fission. Visualized under a modified dark-field microscope, the formation of cannulae, which finally connected all cells, was observed. The cannulae elongated at 1.0 to 1.5 µm/min and reached final lengths of between 30 and 150 µm. A "snapping division"-like mode of cell fission was discovered for Thermoproteus tenax.

* Corresponding author. Mailing address: Archaeenzentrum, Universität Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany. Phone: 49-941-943-3185. Fax: 49-941-943-2403. E-mail: harald.huber{at}biologie.uni-regensburg.de.

Journal of Bacteriology, August 1999, p. 5114-5118, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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