This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brooun, A.
Right arrow Articles by Lewis, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brooun, A.
Right arrow Articles by Lewis, K.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 1999, p. 5131-5133, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Purification and Ligand Binding of EmrR, a Regulator of a Multidrug Transporter

Alexei Brooun, John J. Tomashek, and Kim Lewis*

Biotechnology Center, Tufts University, Medford, Massachusetts 02155

Received 10 May 1999/Accepted 8 June 1999

EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pump---2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p-(trifluoro-methoxy)phenylhydrazone---with affinities in the micromolar range. Equilibrium dialysis experiments suggested one bound ligand per monomer of the dimeric EmrR.

* Corresponding author. Mailing address: Biotechnology Center, Tufts University, 4 Colby St., Medford, MA 02155. Phone: (617) 627-3731. Fax: (617) 627-3993. E-mail: klewis{at}tufts.edu.

Journal of Bacteriology, August 1999, p. 5131-5133, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kumaraswami, M., Schuman, J. T., Seo, S. M., Kaatz, G. W., Brennan, R. G. (2009). Structural and biochemical characterization of MepR, a multidrug binding transcription regulator of the Staphylococcus aureus multidrug efflux pump MepA. Nucleic Acids Res 37: 1211-1224 [Abstract] [Full Text]  
  • Nishino, K., Nikaido, E., Yamaguchi, A. (2007). Regulation of Multidrug Efflux Systems Involved in Multidrug and Metal Resistance of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 189: 9066-9075 [Abstract] [Full Text]  
  • Teran, W., Krell, T., Ramos, J. L., Gallegos, M.-T. (2006). Effector-Repressor Interactions, Binding of a Single Effector Molecule to the Operator-bound TtgR Homodimer Mediates Derepression. J. Biol. Chem. 281: 7102-7109 [Abstract] [Full Text]  
  • Lin, J., Akiba, M., Sahin, O., Zhang, Q. (2005). CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni. Antimicrob. Agents Chemother. 49: 1067-1075 [Abstract] [Full Text]  
  • Wilkinson, S. P., Grove, A. (2004). HucR, a Novel Uric Acid-responsive Member of the MarR Family of Transcriptional Regulators from Deinococcus radiodurans. J. Biol. Chem. 279: 51442-51450 [Abstract] [Full Text]  
  • Loo, T. W., Bartlett, M. C., Clarke, D. M. (2003). Simultaneous Binding of Two Different Drugs in the Binding Pocket of the Human Multidrug Resistance P-glycoprotein. J. Biol. Chem. 278: 39706-39710 [Abstract] [Full Text]  
  • Teran, W., Felipe, A., Segura, A., Rojas, A., Ramos, J.-L., Gallegos, M.-T. (2003). Antibiotic-Dependent Induction of Pseudomonas putida DOT-T1E TtgABC Efflux Pump Is Mediated by the Drug Binding Repressor TtgR. Antimicrob. Agents Chemother. 47: 3067-3072 [Abstract] [Full Text]  
  • Parke, D., Ornston, L. N. (2003). Hydroxycinnamate (hca) Catabolic Genes from Acinetobacter sp. Strain ADP1 Are Repressed by HcaR and Are Induced by Hydroxycinnamoyl-Coenzyme A Thioesters. Appl. Environ. Microbiol. 69: 5398-5409 [Abstract] [Full Text]  
  • Borges-Walmsley, M. I., Beauchamp, J., Kelly, S. M., Jumel, K., Candlish, D., Harding, S. E., Price, N. C., Walmsley, A. R. (2003). Identification of Oligomerization and Drug-binding Domains of the Membrane Fusion Protein EmrA. J. Biol. Chem. 278: 12903-12912 [Abstract] [Full Text]  
  • Grkovic, S., Brown, M. H., Skurray, R. A. (2002). Regulation of Bacterial Drug Export Systems. Microbiol. Mol. Biol. Rev. 66: 671-701 [Abstract] [Full Text]  
  • Godsey, M. H., Zheleznova Heldwein, E. E., Brennan, R. G. (2002). Structural Biology of Bacterial Multidrug Resistance Gene Regulators. J. Biol. Chem. 277: 40169-40172 [Full Text]  
  • Baranova, N., Nikaido, H. (2002). The BaeSR Two-Component Regulatory System Activates Transcription of the yegMNOB (mdtABCD) Transporter Gene Cluster in Escherichia coli and Increases Its Resistance to Novobiocin and Deoxycholate. J. Bacteriol. 184: 4168-4176 [Abstract] [Full Text]  
  • Schumacher, M. A., Miller, M. C., Grkovic, S., Brown, M. H., Skurray, R. A., Brennan, R. G. (2001). Structural Mechanisms of QacR Induction and Multidrug Recognition. Science 294: 2158-2163 [Abstract] [Full Text]  
  • Providenti, M. A., Wyndham, R. C. (2001). Identification and Functional Characterization of CbaR, a MarR-Like Modulator of the cbaABC-Encoded Chlorobenzoate Catabolism Pathway. Appl. Environ. Microbiol. 67: 3530-3541 [Abstract] [Full Text]  
  • Lewis, K. (2001). Riddle of Biofilm Resistance. Antimicrob. Agents Chemother. 45: 999-1007 [Full Text]  
  • Evans, K., Adewoye, L., Poole, K. (2001). MexR Repressor of the mexAB-oprM Multidrug Efflux Operon of Pseudomonas aeruginosa: Identification of MexR Binding Sites in the mexA-mexR Intergenic Region. J. Bacteriol. 183: 807-812 [Abstract] [Full Text]  
  • Putman, M., van Veen, H. W., Konings, W. N. (2000). Molecular Properties of Bacterial Multidrug Transporters. Microbiol. Mol. Biol. Rev. 64: 672-693 [Abstract] [Full Text]  
  • Xiong, A., Gottman, A., Park, C., Baetens, M., Pandza, S., Matin, A. (2000). The EmrR Protein Represses the Escherichia coli emrRAB Multidrug Resistance Operon by Directly Binding to Its Promoter Region. Antimicrob. Agents Chemother. 44: 2905-2907 [Abstract] [Full Text]  
  • Brooun, A., Liu, S., Lewis, K. (2000). A Dose-Response Study of Antibiotic Resistance in Pseudomonas aeruginosa Biofilms. Antimicrob. Agents Chemother. 44: 640-646 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»