This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Raffaelli, N.
Right arrow Articles by Magni, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Raffaelli, N.
Right arrow Articles by Magni, G.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 1999, p. 5509-5511, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Escherichia coli NadR Regulator Is Endowed with Nicotinamide Mononucleotide Adenylyltransferase Activity

Nadia Raffaelli,1 Teresa Lorenzi,1 P. Luigi Mariani,1 Monica Emanuelli,1 Adolfo Amici,1 Silverio Ruggieri,2 and Giulio Magni1,*

Istituto di Biochimica, Facoltà di Medicina1 and Dipartimento di Biotecnologie Agrarie ed Ambientali,2 Università di Ancona, 60131 Ancona, Italy

Received 20 April 1999/Accepted 21 June 1999

The first identification and characterization of a catalytic activity associated with NadR protein is reported. A computer-aided search for sequence similarity revealed the presence in NadR of a 29-residue region highly conserved among known nicotinamide mononucleotide adenylyltransferases. The Escherichia coli nadR gene was cloned into a T7-based vector and overexpressed. In addition to functionally specific DNA binding properties, the homogeneous recombinant protein catalyzes NAD synthesis from nicotinamide mononucleotide and ATP.

* Corresponding author. Mailing address: Istituto di Biochimica, Facoltà di Medicina, Università di Ancona, Via Ranieri, 60131 Ancona, Italy. Phone: (39) 71 2204678. Fax: (39) 71 2802117. E-mail: magnig{at}popcsi.unian.it.

Journal of Bacteriology, September 1999, p. 5509-5511, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gazzaniga, F., Stebbins, R., Chang, S. Z., McPeek, M. A., Brenner, C. (2009). Microbial NAD Metabolism: Lessons from Comparative Genomics. Microbiol. Mol. Biol. Rev. 73: 529-541 [Abstract] [Full Text]  
  • Rodionov, D. A., Li, X., Rodionova, I. A., Yang, C., Sorci, L., Dervyn, E., Martynowski, D., Zhang, H., Gelfand, M. S., Osterman, A. L. (2008). Transcriptional regulation of NAD metabolism in bacteria: genomic reconstruction of NiaR (YrxA) regulon. Nucleic Acids Res 36: 2032-2046 [Abstract] [Full Text]  
  • Rodionov, D. A., De Ingeniis, J., Mancini, C., Cimadamore, F., Zhang, H., Osterman, A. L., Raffaelli, N. (2008). Transcriptional regulation of NAD metabolism in bacteria: NrtR family of Nudix-related regulators. Nucleic Acids Res 36: 2047-2059 [Abstract] [Full Text]  
  • Ostrowski, E. A, Woods, R. J, Lenski, R. E (2008). The genetic basis of parallel and divergent phenotypic responses in evolving populations of Escherichia coli. Proc R Soc B 275: 277-284 [Abstract] [Full Text]  
  • Gerlach, G., Reidl, J. (2006). NAD+ utilization in pasteurellaceae: simplification of a complex pathway.. J. Bacteriol. 188: 6719-6727 [Full Text]  
  • Gerdes, S. Y., Kurnasov, O. V., Shatalin, K., Polanuyer, B., Sloutsky, R., Vonstein, V., Overbeek, R., Osterman, A. L. (2006). Comparative Genomics of NAD Biosynthesis in Cyanobacteria.. J. Bacteriol. 188: 3012-3023 [Abstract] [Full Text]  
  • Berger, F., Lau, C., Dahlmann, M., Ziegler, M. (2005). Subcellular Compartmentation and Differential Catalytic Properties of the Three Human Nicotinamide Mononucleotide Adenylyltransferase Isoforms. J. Biol. Chem. 280: 36334-36341 [Abstract] [Full Text]  
  • Rossolillo, P., Marinoni, I., Galli, E., Colosimo, A., Albertini, A. M. (2005). YrxA Is the Transcriptional Regulator That Represses De Novo NAD Biosynthesis in Bacillus subtilis. J. Bacteriol. 187: 7155-7160 [Abstract] [Full Text]  
  • Merdanovic, M., Sauer, E., Reidl, J. (2005). Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae. J. Bacteriol. 187: 4410-4420 [Abstract] [Full Text]  
  • Grose, J. H., Bergthorsson, U., Xu, Y., Sterneckert, J., Khodaverdian, B., Roth, J. R. (2005). Assimilation of Nicotinamide Mononucleotide Requires Periplasmic AphA Phosphatase in Salmonella enterica. J. Bacteriol. 187: 4521-4530 [Abstract] [Full Text]  
  • Grose, J. H., Bergthorsson, U., Roth, J. R. (2005). Regulation of NAD Synthesis by the Trifunctional NadR Protein of Salmonella enterica. J. Bacteriol. 187: 2774-2782 [Abstract] [Full Text]  
  • Sauer, E., Merdanovic, M., Price Mortimer, A., Bringmann, G., Reidl, J. (2004). PnuC and the Utilization of the Nicotinamide Riboside Analog 3-Aminopyridine in Haemophilus influenzae. Antimicrob. Agents Chemother. 48: 4532-4541 [Abstract] [Full Text]  
  • Saridakis, V., Pai, E. F. (2003). Mutational, Structural, and Kinetic Studies of the ATP-binding Site of Methanobacterium thermoautotrophicum Nicotinamide Mononucleotide Adenylyltransferase. J. Biol. Chem. 278: 34356-34363 [Abstract] [Full Text]  
  • Miller, E. S., Heidelberg, J. F., Eisen, J. A., Nelson, W. C., Durkin, A. S., Ciecko, A., Feldblyum, T. V., White, O., Paulsen, I. T., Nierman, W. C., Lee, J., Szczypinski, B., Fraser, C. M. (2003). Complete Genome Sequence of the Broad-Host-Range Vibriophage KVP40: Comparative Genomics of a T4-Related Bacteriophage. J. Bacteriol. 185: 5220-5233 [Abstract] [Full Text]  
  • Kurnasov, O. V., Polanuyer, B. M., Ananta, S., Sloutsky, R., Tam, A., Gerdes, S. Y., Osterman, A. L. (2002). Ribosylnicotinamide Kinase Domain of NadR Protein: Identification and Implications in NAD Biosynthesis. J. Bacteriol. 184: 6906-6917 [Abstract] [Full Text]  
  • Singh, S. K., Kurnasov, O. V., Chen, B., Robinson, H., Grishin, N. V., Osterman, A. L., Zhang, H. (2002). Crystal Structure of Haemophilus influenzae NadR Protein. A BIFUNCTIONAL ENZYME ENDOWED WITH NMN ADENYLYLTRANSFERASE AND RIBOSYLNICOTINAMIDE KINASE ACTIVITIES. J. Biol. Chem. 277: 33291-33299 [Abstract] [Full Text]  
  • Gerdes, S. Y., Scholle, M. D., D'Souza, M., Bernal, A., Baev, M. V., Farrell, M., Kurnasov, O. V., Daugherty, M. D., Mseeh, F., Polanuyer, B. M., Campbell, J. W., Anantha, S., Shatalin, K. Y., Chowdhury, S. A. K., Fonstein, M. Y., Osterman, A. L. (2002). From Genetic Footprinting to Antimicrobial Drug Targets: Examples in Cofactor Biosynthetic Pathways. J. Bacteriol. 184: 4555-4572 [Abstract] [Full Text]  
  • Kemmer, G., Reilly, T. J., Schmidt-Brauns, J., Zlotnik, G. W., Green, B. A., Fiske, M. J., Herbert, M., Krai{beta}, A., Schlor, S., Smith, A., Reidl, J. (2001). NadN and e (P4) Are Essential for Utilization of NAD and Nicotinamide Mononucleotide but Not Nicotinamide Riboside in Haemophilus influenzae. J. Bacteriol. 183: 3974-3981 [Abstract] [Full Text]  
  • Mehl, R. A., Kinsland, C., Begley, T. P. (2000). Identification of the Escherichia coli Nicotinic Acid Mononucleotide Adenylyltransferase Gene. J. Bacteriol. 182: 4372-4374 [Abstract] [Full Text]  
  • Emanuelli, M., Carnevali, F., Saccucci, F., Pierella, F., Amici, A., Raffaelli, N., Magni, G. (2001). Molecular Cloning, Chromosomal Localization, Tissue mRNA Levels, Bacterial Expression, and Enzymatic Properties of Human NMN Adenylyltransferase. J. Biol. Chem. 276: 406-412 [Abstract] [Full Text]  
  • Saridakis, V., Christendat, D., Kimber, M. S., Dharamsi, A., Edwards, A. M., Pai, E. F. (2001). Insights into Ligand Binding and Catalysis of a Central Step in NAD+ Synthesis. STRUCTURES OF METHANOBACTERIUM THERMOAUTOTROPHICUM NMN ADENYLYLTRANSFERASE COMPLEXES. J. Biol. Chem. 276: 7225-7232 [Abstract] [Full Text]  
  • Olland, A. M., Underwood, K. W., Czerwinski, R. M., Lo, M.-C., Aulabaugh, A., Bard, J., Stahl, M. L., Somers, W. S., Sullivan, F. X., Chopra, R. (2002). Identification, Characterization, and Crystal Structure of Bacillus subtilis Nicotinic Acid Mononucleotide Adenylyltransferase. J. Biol. Chem. 277: 3698-3707 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»