Journal of Bacteriology, September 1999, p. 5539-5541, Vol. 181, No. 17
Department of Biology, University of Utah,
Salt Lake City, Utah 84112,1 and
Department of Biochemistry and Molecular Genetics, University
of Virginia Health Sciences Center, Charlottesville, Virginia
229082
Received 3 May 1999/Accepted 18 June 1999
No periplasmic binding protein has been demonstrated for the
ATP-binding cassette (ABC)-type cobalamin transporter BtuCD. New
mutations (btuF) are described that affect inner-membrane transport. The BtuF protein has a signal sequence and resembles the
periplasmic binding proteins of several other ABC transporters.
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
A New Class of Cobalamin Transport Mutants
(btuF) Provides Genetic Evidence for a Periplasmic Binding
Protein in Salmonella typhimurium
*
Corresponding author. Mailing address: Department of
Biology, University of Utah, Salt Lake City, UT 84112. Phone: (801)
581-3412. Fax: (801) 585-6207. E-mail:
Roth{at}Bioscience.utah.edu.
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