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Journal of Bacteriology, September 1999, p. 5652-5661, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genomic Subtraction Identifies Salmonella typhimurium Prophages, F-Related Plasmid Sequences, and a Novel Fimbrial Operon, stf, Which Are Absent in Salmonella typhi

Melanie Emmerth,1 Werner Goebel,1 Samuel I. Miller,2 and Christoph J. Hueck3,*

Lehrstuhl für Mikrobiologie, Biozentrum der Universität Würzburg, 97074 Würzburg,1 and CREATOGEN GmbH, 86156 Augsburg,3 Germany, and Department of Microbiology, University of Washington, Seattle, Washington 981952

Received 19 January 1999/Accepted 25 June 1999

Salmonella typhimurium causes systemic and fatal infection in inbred mice, while the related serotype Salmonella typhi is avirulent for mammals other than humans. In order to identify genes from the virulent strain S. typhimurium ATCC 14028 that are absent in S. typhi Ty2, and therefore might be involved in S. typhimurium mouse virulence, a PCR-supported genomic subtractive hybridization procedure was employed. We have identified a novel putative fimbrial operon, stfACDEFG, located at centisome 5 of the S. typhimurium chromosome, which is absent in S. typhi, Salmonella arizonae, and Salmonella bongori but was detected in several other Salmonella serotypes. The fimbrial genes represent a genomic insertion in S. typhimurium compared to the respective region between fhuB and hemL in Escherichia coli K-12. In addition, the subtraction procedure yielded F plasmid-related sequences from the S. typhimurium virulence plasmid, a number of DNA fragments representing parts of lambdoid prophages and putative sugar transporters, and several fragments with unknown sequences. The majority of subtracted chromosomal sequences map to three distinct locations, around centisomes 5, 27, and 57.

* Corresponding author. Mailing address: CREATOGEN GmbH, Ulmer Strasse 160a, 86156 Augsburg, Germany. Phone: (49) 821-444650. Fax: (49) 821-4446529. E-mail: cjh{at}creatogen.de.

Journal of Bacteriology, September 1999, p. 5652-5661, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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