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Journal of Bacteriology, September 1999, p. 5718-5724, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of sigma B-Dependent Genes in Bacillus subtilis Using a Promoter Consensus-Directed Search and Oligonucleotide Hybridization

Anja Petersohn,1 Jörg Bernhardt,1 Ulf Gerth,1 Dirk Höper,1 Torsten Koburger,1 Uwe Völker,2 and Michael Hecker1,*

Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, 17487 Greifswald,1 and Laboratorium für Mikrobiologie und Max-Planck-Institut für terrestrische Mikrobiologie, Philipps-Universität Marburg, 35043 Marburg,2 Germany

Received 23 February 1999/Accepted 23 June 1999

A consensus-directed search for sigma B promoters was used to locate potential candidates for new sigma B-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations with total RNA from exponentially growing or ethanol-stressed cells of the wild type as well as a sigB mutant revealed 22 genes that required sigma B for induction by ethanol. Although almost 50% of the proteins encoded by the newly discovered sigma B-dependent stress genes seem to be membrane localized, biochemical functions have so far not been defined for any of the gene products. Allocation of the genes to the sigma B-dependent stress regulon may indicate a potential function in the establishment of a multiple stress resistance. AldY and YhdF show similarities to NAD(P)-dependent dehydrogenases and YdbP to thioredoxins, supporting our suggestion that sigma B-dependent proteins may be involved in the maintenance of the intracellular redox balance after stress.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, Friedrich-Ludwig-Jahn-Straße 15, 17487 Greifswald, Germany. Phone: 49-3834-864200. Fax: 49-3834-864202. E-mail: hecker{at}microbio7.biologie.uni-greifswald.de.

Journal of Bacteriology, September 1999, p. 5718-5724, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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