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Journal of Bacteriology, October 1999, p. 6028-6032, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Nitrite and Nitrous Oxide Reductase Regulation by Nitrogen Oxides in Rhodobacter sphaeroides f. sp. denitrificans IL106

Monique Sabaty,* Carole Schwintner,dagger Sandrine Cahors, Pierre Richaud, and Andre Verméglio

Commissariat à l'Énergie Atomique/Cadarache DSV, DEVM, Laboratoire de Bioénergétique Cellulaire, 13108 St. Paul lez Durance Cedex, France

Received 16 February 1999/Accepted 19 July 1999

We have cloned the nap locus encoding the periplasmic nitrate reductase in Rhodobacter sphaeroides f. sp. denitrificans IL106. A mutant with this enzyme deleted is unable to grow under denitrifying conditions. Biochemical analysis of this mutant shows that in contrast to the wild-type strain, the level of synthesis of the nitrite and N2O reductases is not increased by the addition of nitrate. Growth under denitrifying conditions and induction of N oxide reductase synthesis are both restored by the presence of a plasmid containing the genes encoding the nitrate reductase. This demonstrates that R. sphaeroides f. sp. denitrificans IL106 does not possess an efficient membrane-bound nitrate reductase and that nitrate is not the direct inducer for the nitrite and N2O reductases in this species. In contrast, we show that nitrite induces the synthesis of the nitrate reductase.

* Corresponding author. Mailing address: CEA/Cadarache DSV, DEVM, Laboratoire de Bioénergétique Cellulaire, Bât. 156, 13108 St. Paul lez Durance Cedex, France. Phone: 33 4 42 25 35 70. Fax: 33 4 42 25 47 01. E-mail: msabaty{at}cea.fr.

dagger Present address: Laboratoire de Génétique Moléculaire de la Recombinaison, Service Recherche, Institut Curie, 26 rue d'Ulm, 75231 Paris Cedex, France.

Journal of Bacteriology, October 1999, p. 6028-6032, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Rich, J. J., Heichen, R. S., Bottomley, P. J., Cromack, K. Jr., Myrold, D. D. (2003). Community Composition and Functioning of Denitrifying Bacteria from Adjacent Meadow and Forest Soils. Appl. Environ. Microbiol. 69: 5974-5982 [Abstract] [Full Text]  
  • Sabaty, M., Avazeri, C., Pignol, D., Vermeglio, A. (2001). Characterization of the Reduction of Selenate and Tellurite by Nitrate Reductases. Appl. Environ. Microbiol. 67: 5122-5126 [Abstract] [Full Text]  


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